芍药甘草汤对子宫腺肌病细胞ERK1 mRNA/ERK2 mRNA的影响被引量：3

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摘要目的:通过观察芍药甘草汤对子宫腺肌病痛经患者的病灶细胞ERK1 mRNA/ERK2 mRNA表达水平的影响,探讨芍药甘草汤对子宫腺肌病痛经治疗的深层作用机理。方法:实验分为化瘀止痛方高、低剂量组,空白对照组,基质组及西药对照米非司酮组(RU486)组,每组5例,采用胶原酶消化法培养人子宫腺肌病病灶细胞,采用荧光定量PCR检测药物干预前后子宫腺肌病病灶细胞ERK1 mRNA/ERK2 mRNA表达的变化。结果:与空白对照组相比,芍药甘草高低浓度组细胞ERK2mRNA表达均有不同程度的下降,差异有统计学意义(P<0.05),而细胞ERK1mRNA表达差异无统计学意义(P>0.05)。结论:芍药甘草汤对于子宫腺肌病痛经的治疗机理可能在于其对于ERK2 mRNA的抑制,从而减缓了子宫平滑肌的收缩强度,子宫收缩性减弱,有效缓解了患者的痛经症状,此可能是芍药甘草汤治疗子宫腺肌病的主要机理之一。

作者关永格何昱雯李坤寅

机构地区广州中医药大学第一附属医院东莞市大岭山医院广州中医药大学

出处《四川中医》 2014年第7期60-62,共3页 Journal of Sichuan of Traditional Chinese Medicine

基金国家自然科学基金(No.81173296) 广东省普通高校"中医女性生殖调节与安全性研究"重点实验室开放基金资助项目(No.ZF201107)

关键词芍药甘草汤子宫腺肌病痛经 ERK1 MRNA ERK2 MRNA

分类号 R285.5 [医药卫生—中药学]

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