温度和时间对血凝试验测定禽流感病毒H5N1亚型Re-6株抗原效价的影响

Effects of Temperature and Time on the Antigen Titer of H5N1 Subtype of Avian Influenza Re-6 Strain by Hemagglutination Test

旨在研究不同温度和反应时间对血凝试验测定禽流感病毒H5N1亚型Re-6株抗原效价的影响。试验设定4、25、37℃3个温度,分别读取反应5、10、15、20、25、30、35、40、45、50、55、60min时的抗原效价。结果表明,随着反应温度的升高,试验结果出现时间缩短。4℃反应25 min结果趋于稳定,反应50 min时开始解凝;25℃反应20 min结果趋于稳定,反应40 min时开始解凝;37℃反应15 min结果趋于稳定,反应30 min时开始解凝。

The research aimed to study the effects of different temperature and reaction time on the antigen titer of H5N1 subtype of avian influenza Re-6 strain determined by hemagglutination test. Three temperatures （4, 25 and 37℃） were set to read the titers after reacting 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 and 60 min respectively. The results showed that the the appearance time of result was shortened with the increase of reaction temperature. The results tended to be stable after reacting at 4℃for 25 min and deflocculation started after reacting 50 min. The results tended to be stable after reacting at 25 ℃ for 20 min and deflocculation started after reacting 40 min. The results tended to be stable after reacting at 37 ℃for 15 min and deflocculation started after reacting 30 min.