LC-MS/MS法测定烟用纸张中的五氯苯酚

Determination of Pentachlorophenol in Papers for Cigarette Product by LC-MS/MS Method

建立了测定烟用纸张中五氯苯酚的液相色谱-串联质谱法（LC-MS/MS）.烟用纸张样品超声提取后直接进样,在Atiantis d C18柱上以甲醇-水（含0.1％甲酸）为流动相进行分离,用LC-MS/MS在多反应监测（MRM）模式下测定,内标法定量.结果表明：①方法的线性范围是1～20 ng/mL,线性关系良好（R2 ＞0.99）,平均回收率＞91.6％,相对标准偏差（RSD）＜8％（n=5）,定量限为0.018 mg/kg.②样品前处理过程简单,无需复杂的衍生化操作.建立的方法适用于烟用纸张中五氯苯酚的快速检测.

A method based on high performance liquid chromatography-tandem mass spectrometry （LC-MS/MS） was developed for determining the pentachlorophenol in cigarette papers.The sample of paper was ultrasonically extracted,the extract was directly injected into and separated on an Atiantis d C18 column by using methanol-water （containing 0.1％ formic acid） as mobile phase.The pentachlorophenol was detected by LC-MS/MS under multiple reaction monitoring （MRM） mode and quantified by internal standard method.The results showed that：1） The linear range and quantification limit of this method were 1-20 ng/mL and 0.018 mg/kg,respectively.The linear relation was good （R2＞0.99）.The average recoveries were more than 91.6％ with the relative standard deviations less than 8％.2） This method was suitable for the fast analysis of pentachlorophenol in papers for cigarette product with the advantages of simple pretreatment and free of complex derivation steps.