徐州地区汉族RhD阴性人群RHD基因多态性研究

Study of RHDgenes polymorphism among RhD negative Han indivduals in Xuzhou area

目的研究徐州地区汉族RhD阴性人群RHD基因多态性。方法采用常规血清学技术检测RhD抗原表型,采用抗球蛋白试验(IAT)确认结果。采用序列特异性引物-聚合酶链反应(SSP-PCR)方法检测RHD基因。结果 110例RhD阴性个体中,SSP-PCR检测RHD基因结果为RHD阳性基因携带者5例,RHD阴性基因携带者47例,RHD-CE(2-9)-D基因携带者22例,DVIⅢ型基因携带者17例,弱D15基因携带者2例,DEL-1227A基因携带者17例。结论徐州地区汉族人群RhD阴性个体呈现RHD基因结构复杂的多态性,以变异体等位基因RH-CE(2-9)-D基因、DVIⅢ型基因、DEL-1227A基因为主。

Objective To investigate the RHD gene polymorphism among the RhD negative Han individuals in Xuzhou.Methods The RhD antigen phenotypes were detected by routine serological techniques,and were identified with indirect antiglobulin test （IAT）.The genotypes of RHD were detected by using sequence specific primer polymerase chain reaction（SSP-PCR）.Results A-mong 110 RhD negative individuals,there were 5 cases carring RHD positive gene,47 cases carring RHD negative gene,22 cases carring RHD-CE（2-9）-D gene,17 cases carring DVI Ⅲ gene,2 cases carring weak D15 gene,and 17 carring DEL-1227A gene,re-spectively.Conclusion The RhD negative Han individuals present complex RHD gene polymorphism in Xuzhou region,and variant alleles such as RHD-CE（2-9）-D,DVI Ⅲ,DEL-1227A are given priority.