成分输血对儿童急性白血病免疫功能的影响

Effect of transfusion of blood components on immune function in children with acute leukemia

目的探讨成分输血对儿童急性白血病免疫功能的影响。方法收集36例急性白血病患儿输血前、后的血清标本,依据不同输血剂量分组,采用酶联免疫吸附测定法(ELISA)检测细胞因子白细胞介素(IL)-2、IL-4、IL-10的表达变化。结果输血前各组之间IL-2的表达无显著差异(P>0.05)。输血后小剂量输血组患儿的IL-2未明显改变(P>0.05);大剂量输血组患儿的IL-2表达降低,差异有显著性(P<0.05);大、小剂量输血组患儿的IL-4表达较输血前明显降低(P<0.05);输血后大剂量输血组患儿的IL-4表达降低更加显著,具有显著差异(P<0.05);输血前大剂量输血组患儿的IL-10表达明显增加(P<0.05),而小剂量输血组患儿的IL-10表达变化不明显(P>0.05);输血后大、小剂量输血组患儿的IL-10表达较输血前明显增加(P<0.05)。结论不同剂量的成分输血可改变急性白血病患儿的免疫功能,应高度重视急性白血病患儿的输血方案及最佳输血剂量的选择。

Objective To investigate the effect of transfusion of blood components on immune function in children with acute leukemia. Method Serum samples were collected from 36 cases of acute leukemia patients before and after transfusion. They were divided into 2 groups according to different dose of transfusion. ELISA method was used for the detection of cytokines IL-2, IL-4, IL-10 expression. Result The expression of IL-2 had no statistical difference between the groups before blood transfusion（P〉0.05）. IL-2 in low-dose group didn＇t change significantly after transfusion（P〉0.05）; IL-2 in high-dose group decreased, which had significant difference after transfusion（P〈0.05）. IL-4 in both groups decreased obviously before transfusion（P〈0.05）. IL-4 in high-dose group reduced more significantly after transfusion（P〈0.05）. Before transfusion, IL-10 in high-dose group increased significantly（P〈0.05）, while the expression of IL-10 in low-dose group didn＇t change obviously（P〉0.05）. After transfusion, IL-10 in both groups increased more significantly（P〈0.05）. Conclusion Different dose of transfusion with blood components can affect the immune function. Therefore, the transfusion dose selection should be paid more attention for therapy of children with acute leukemia.