摘要
A novel method for rapid,accurate and nondestructive determination of trimethoprim in complex matrix was presented.Near-infrared spectroscopy coupled with multivariate calibration（partial least-squares and artificial neural networks） was applied in the experiment.The variable selection process based on a modified genetic algorithm with fixed number of selected variables was proceeded,which can reduce the training time and enhance the predictive ability when coupled with artificial neural network model.
A novel method for rapid,accurate and nondestructive determination of trimethoprim in complex matrix was presented.Near-infrared spectroscopy coupled with multivariate calibration（partial least-squares and artificial neural networks） was applied in the experiment.The variable selection process based on a modified genetic algorithm with fixed number of selected variables was proceeded,which can reduce the training time and enhance the predictive ability when coupled with artificial neural network model.
基金
Supported by the National Natural Science Foundation of China（No.21207047）, the State Major Project for Science and Technology Development, China（No.2011YQ14015001）, the Basic Research Foundation of Jilin University, China （Nos.201103096, 201103102） and the Science-Technology Development Project of Jilin Province of China（Nos.201105008, 20126018, 20130206014GX）.
