应用体外仿生模型分析南极磷虾中氟的生物可给性及其对人体的健康风险

Bioaccessibility analysis and health risk assessment of fluorine in Euphausia superba using in vitro whole- bionic model

运用体外全仿生消化模型研究了南极磷虾中氟的生物可给性及其对人体的健康风险.采用氟离子选择电极法进行分析,结果表明,南极磷虾中氟的溶解态含量及其生物可给性差异很大,模拟胃液中氟的溶解态含量为81.68～1 987.4 mg/kg,生物可给性为61.24％～ 87.50％;模拟小肠液中氟的溶解态含量为23.48～ 550.64 mg/kg,生物可给性为16.97％ ～27.31％.胃液中氟的溶解态含量与南极磷虾是否带壳有关.如以胃消化阶段判断,摄入南极磷虾中氟对儿童的日允许摄入量（TDI）贡献率均低于1.0％,其中虾肉的最低为0.035％.如以小肠消化阶段判断,摄入南极磷虾中氟对儿童的TDI贡献率均低于0.30％.研究还发现,经过不同加工处理的南极磷虾摄入的氟对人体均没有太大的风险,若食用去壳后的南极磷虾且日摄入量控制在8000 mg以内,其安全性更高.本研究为科学评价南极磷虾中氟的食用安全性和有效提高南极磷虾的加工利用提供参考.

In vitro whole - bionic assessment in Euphausia superba. digestion model was used to study the bioaccessibility of fluorine and its health risk Fluorine content was determined by ion selection election. The results showed that a great difference existed in different extracts for the dissolved fluorine content and its bioavailability. The dissolved fluorine content in the gastric extracts was between 81.68 and 1 987.4 mg/kg, and its bioaccessibility was between 61. 24% and 87. 50%. The dissolved fluorine content in the intestinal extracts was between 23.48 and 550.64 mg/kg, and its bioaccessibility was between 16.97% and 27.31%. The fluorine content in the gastric extracts was related to the shrimp shell. Considering the fluorine content and bioavailability in the gastric extracts, the contribution rate of fluorine in E. superba to the children tolerable daily intake （TDI） was below 0. 035%, and if considering the fluorine content and bioavailability in the intestinal extracts, the contribution rate of fluorine in E. superba to the children TDI was below 0.30%. So, the human health risk of fluorine in E. superba with different processing method as in the present experiment was low. If the daily intake content of the shrimp without shell doesn＇ t exceed 8 000 mg, it is safe for an average person. The present result provides important information for the safety evaluation of fluoride in E. superba, and the deep processing and the utilization of E. superba.