糖心乐对糖尿病心肌病大鼠心肌酶及心肌转化生长因子β_1的影响

Effects of Tangxinle on myocardial enzyme and transforming growth factor-β_1 in rats with diabetic cardiomyopathy

目的探讨糖心乐(TXL)对糖尿病心肌病大鼠心肌酶、心肌转化生长因子β1(TGF-β1)表达的影响。方法SD大鼠腹腔注射链脲佐菌素建立糖尿病模型,8周后形成糖尿病心肌病模型,随机分为模型对照组、达美康对照组、TXT大、小剂量组,各组大鼠均为12只,另设正常SD大鼠10只作为正常对照组。达美康对照组、TXL大、小剂量组分别给予3 mg/mL达美康混悬液、2 g/mL TXL、1 g/mL TXL灌胃治疗,模型对照组给予生理盐水灌胃。治疗4周后处死,取血检测血糖、肌酸激酶(CK)、乳酸脱氢酶(LDH),切取心肌组织,免疫组化法检测TGF-β1表达情况。结果与模型对照组比较,TXL大剂量组血糖明显降低[(10.54±2.06)mmol/L比(23.16±5.74)mmol/L],CK、LDH明显升高[(120.57±18.83)U/mg比(78.26±12.01)U/mg,(5052.06±418.64)U/mg比(3784.15±349.62)U/mg];TGF-β1阳性染色颗粒的平均光密度(MOD)降低[(0.148±0.006)比(0.262±0.008)];差异均有高度统计学意义(均P<0.01)。结论 TXL对糖尿病大鼠有一定的降血糖作用,可减少心肌酶漏出,改善心肌能量代谢,同时降低心肌中TGF-β1的表达,减慢心肌组织纤维化进程,延缓和改善糖尿病心肌病的心肌损伤。

Objective To explore the effects of Tangxinle （TXL） on myocardial enzyme and transforming growth factor-β1 （TGF-β1） in experimental rats with diabetic cardiomyopathy. Methods Diabetes model was established with streptozotocin by intraperitoneal injection in Sprague-Dawley rats, after 8 weeks diabetes cardiomyopathy model was formed, then diabetic cardiomyopathy rats were randomly divided into four groups： model control group, the Diamicron control group, TXL high-dose group, TXL small-dose group, 12 rats in each group, 10 normal rats were as the normal control group. Diamicron control group, TXL high-dose group, TXL small-dose group were given 3 mg/mL of Diamicron suspension, 2 g/mL TXL, 1 g/mL TXL lavage treatment respectively, the model control group was given normal saline lavage. Rats of five groups were killed after 4 weeks treatment. Blood glucose, creatine kinase （CK） and lactic dehydrogenase （LDH） were detected, and the expression of TGF-β1 in five groups were detected by immunohistochemistry method. Results Compared with model control group, TXL high-dose group fasting blood glucose decreased obviously [（10.54±2.06） mmol/L vs （23.16±5.74） mmol/L];CK and LDH increased remarkably [（120.57±18.83） U/mg vs （78.26±12.01） U/mg;（5052.06±418.64） U/mg vs （3784.15±349.62） U/mg];the average absorbance value of staining positive particles TGF-β1 in myocardial cell reduced [（0.148±0.006） vs （0.262±0.008）], the differences were statistically significant （all P〈0.01）. Conclusion TXL has a certain hypoglycemic effect on diabetic rats, it can reduce the myocardial enzyme leakage to improve the metabolic disorder of energy, meanwhile it can reduce the expression of TGF-β1 to slow down fibrous degeneration in myocardial tissue and delay development of diabetic cardiomyopathy.