摘要
[目的]了解光照时间在黄芩Scutellaria baicalensis Georgi种子萌发过程中对初生与次生代谢规律的影响。[方法]光合色素、可溶性糖、苯丙氨酸解氨酶(PAL)和肉桂酸-4-羟化酶活性测定采用紫外分光光度法。次生代谢物质含量测定采用高效液相色谱法。[结果]黄芩种子在萌发过程中对光照时间不敏感,黄芩种苗的生长对光照时间是非常敏感的,光合色素、可溶性糖、苯丙氨酸解氨酶(PAL)和肉桂酸-4-羟化酶活性均随着光照强度增强而增加,其次生代谢物质也呈现出相同的变化趋势。[结论]光照时间促进了光合色素的形成,进而促进了初生代谢物质、关键酶活性和次生代谢物质的合成,因此我们可以通过调节光照时间来提高黄芩药材的质量。
This study aimed to investigate the primary and secondary metabolism of Scutel aria baicalensis Georgi during seed germination process under different il umination time. [Method] Chlorophyl （CHL） content, soluble sugar content, phenylalanine ammonia lyase （PAL） activity and cinnamate-4-hydroxylase （C4H） ac-tivity were determined with ultraviolet spectrophotometry. The secondary metabolites were detected by high performance liquid chromatography （HPLC）. [Result] The re-sults indicated that the germination of S. baicalensis seeds was not sensitive to light and the seedlings were very sensitive to light. CHL content, soluble sugar content, PAL activity and C4H activity increased continuously with the il umination time. The secondary metabolites showed a similar trend. [Conclusion] Il umination time promoted the formation of leaf photosynthetic pigments, thereby affecting the synthesis of primary and secondary metabolites and the activities of PAL and C4H. Therefore, the quality of S. baicalensis can be improved by regulating the il umina-tion time appropriately.
基金
Supported by Agricultural Improved Variety Project of Shandong Province(2005LZ08,2008LZ013)~~
