头痛宁胶囊对偏头痛大鼠中脑降钙素基因相关肽表达的影响

Effects of Toutongning Capsule on CGRP expression in the midbrain of rats with migraine headache model

目的探讨头痛宁胶囊干预偏头痛大鼠,对模型大鼠中脑降钙素基因相关肽(CGRP)基因表达的影响,及头痛宁胶囊对内源性痛觉调制系统功能的干预作用。方法将24只健康成年Wistar大鼠随机分为对照组(A组),偏头痛组(B组),头痛宁胶囊对照组(C组),头痛宁胶囊治疗组(D组),每组6只。C、D组给予头痛宁胶囊0.375 g/(kg·d)灌胃7 d,之后B、D组大鼠制备硝酸甘油型偏头痛大鼠模型,造模2 h处死大鼠,取中脑组织。采用实时定量PCR检测各组大鼠中脑CGRP mRNA拷贝数。结果 A、B、C、D组大鼠中脑每250 ng总RNA中脑CGRP mRNA拷贝数分别为:(1.13±0.68)×104、(1.61±0.51)×104、(0.80±0.45)×104、(0.75±0.27)×104。A组与B组、C组与D组大鼠中脑CGRP mRNA拷贝数比较,差异均无统计学意义(P>0.05);C、D组大鼠中脑CGRP mRNA拷贝数明显低于B组,差异有统计学意义(P<0.05)。结论头痛宁胶囊可以降低偏头痛发作时中脑CGRP基因的表达,发挥治疗偏头痛的作用。

Objective To investigate the effects of Toutongning Capsule on Calcitonin gene-related peptide （CGRP） ex-pression in the midbrain of rats with migraine headache, and the intervention effect of Toutongning Capsule on the function of endogenous pain modulation system. Methods A total of 24 adult Wistar rats were divided into control group （group A）, model group （group B）, Toutongning Capsule control group （group C） and Toutongning Capsule treat-ment group （group D）, with 6 rats in each group. Group C and group D were given the Toutongning Capsule 0.375 g/（kg＆#183;d） for 7 days. 2 hours following nitroglycerin injection, the midbrains of rats were isolated and the expression of CGR was detected by real-time quantitative PCR. Results CGRP mRNA levels （CGRP mRNA copies per 250 ng total RNA） in the rat midbrain of group A, B, C, D were （1.13±0.68）×104, （1.61±0.51）×104, （0.80±0.45）×104, （0.75±0.27）×104 respec-tively. CGRP mRNA levels were similar between group A and group B, group C and group D, there were no significant differences in the CGRP mRNA copy number in the rat midbrain （P〉0.05）. The CGRP mRNA levels were significant-ly lower in the group C and group D compared with the groups B, the difference was statistically significant （P〈0.05）. Conclusion The therapeutically beneficial effects of Toutongning Capsule to migraine headaches is ralated to downreg-ulation of calcitonin gene-related peptide mRNA expressions in the midbrain.