血液病患者外周血白细胞、血浆和血清中EB病毒DNA定量分析

Quantitative analysis on EB virus DNA in peripheral white blood cell,plasma and serum in patients with hematologic diseases

目的比较血液病患者外周血白细胞、血浆和血清的EB病毒载量,探讨应用患者血清或者血浆检测EB病毒载量的可行性。方法采集125例血液病患者的静脉血,分别进行外周血白细胞、血浆和血清的分离,应用荧光定量PCR技术检测三种标本中病毒载量,以外周血白细胞EB病毒载量为金标准,血浆和血清检测结果与之比较,进行分析。结果血浆和血清的EB病毒载量与外周血白细胞病毒载量相比较差异无统计学意义(P>0.05)。结论应用患者血浆或血清进行血液EBV DNA定量检测,将成为代替外周血白细胞的一种可靠方法。

Objective To compare the load of EB virus in peripheral white blood cell,plasma and serum in the patients with he-matologic diseases,and to discuss the feasibility of detecting EB virus load by using plasma or serum instead of peripheral white blood cell.Methods Venous blood of 125 patients with hematologic diseases were collected,and peripheral white blood cell,plasma and serum were isolated.The real-time quantitative PCR was used to detect the virus load in three kinds samples with the EB virus load in peripheral blood cell as the gold standard.Results Compared to peripheral white blood,the EB virus load in plasma and ser-um showed no statistical difference（P〉0.05）.Conclusion Using plasma or serum instead of peripheral white blood cell for con-ducting the quantitative detection of the load of EB virus will be a reliable method.