荧光定量PCR法检测乙肝病毒DNA和乙肝病毒标志物检测的临床价值分析

The clinical value of detection of fluorescence quantitative PCR in detection of HBV DNA and HBV markers

目的：探讨荧光定量 PCR 法（FQ-PCR）检测乙肝病毒 DNA（HBV-DNA）和乙肝病毒标志物（HBV-M）ELISA 法检测的临床价值。方法选取2013年4～12月在该院检测的653例乙肝患者，先采用 ELISA 法对其血液标本进行 HBV-M 模式定性检测，检测顺序为乙型肝炎病毒表面抗原（HBsAg）、乙肝表面抗体（HbsAb）、乙型肝炎 e 抗原（HbeAg）、乙型肝炎 E 抗体（Hbe-Ab）、乙肝表面核心抗体（HbcAb）；再采用 FQ-PCR 法进行 HBV-DNA 定量检测，观察不同 HBV-M 模式检测结果。结果 HB-sAg、HbeAg、HbcAb 检测同时阳性简称“大三阳”。大三阳[1（＋）、3（＋）、5（＋）模式]和[1（＋）、3（＋）模式]的 HBV-DNA 阳性率分别为97．97％、94．74％，显著高于其他模式的 HBV-DNA 阳性率（P ＜0．05）。大三阳的 HBV-DNA 表达水平（5．59×10^6±2．42×10^5）copies/mL，明显高于其他模式（P ＜0．05）。结论联合 HBV-M 定性及 HBV-DNA 定量检测，对临床早期诊断及用药具有重要指导价值。

Objective To investigate the clinical value of detection of fluorescence quantitative PCR in detection of HBV DNA and HBV markers.Methods 653 hepatitis B patients in our hospital from 2013 April to 2013 December were selected.First ELISA method using HBV-M model for qualitative detection of the blood samples,the detection order：Hepatitis b virus surface antigen （HBsAg）,Hepatitis B surface Antibody（HbsAb）,Hepatitis B e Antigen（HbeAg）,hepatitis Be antibody（HbeAb）,hepatitis B core antibody（HbcAb）;Then the FQ-PCR method for the quantitative detection of HBV-DNA,different HBV-m model test results were compared..Results Big 3 this world[1（＋）、3 （＋）、5 （＋）model]and[1 （＋）、3 （＋）model ]of the HBV-DNA positive rate was 97.97%,94.74% was significantly higher than that in other mode（P〈0.05）.Big 3 this world[1（＋）、3（＋）、5（＋）model]HBV-DNA expression level is the highest（5.59 ×10^6 ±2.42×10^5 ）copies/mL was significantly higher than that in other mode（P〈0.05）.Conclusion Combined with qualitative and quantitative detection of HBV-DNA HBV-M,which is of great value to clinical early diagnosis and therapy.