抗DNA酶B对小儿链球菌感染相关疾病的价值

The value of anti-DNA enzyme B on related diseases in children with Streptococcus infection

目的：探讨抗DNA酶B对小儿链球菌感染相关疾病的价值。方法收集本院2012年1月～2013年10月诊断为链球菌感染的患儿114例,将其分为急性风湿热组、急性咽炎和扁桃体炎组、急性肾小球肾炎组、风湿性心脏病组,同时选取健康儿童30例为对照组。用微量中和法检测抗DNA酶B,特定蛋白仪免疫散射比浊法检测抗链球菌溶血素O（ASO）,并同时检测咽拭子培养、C反应蛋白（CRP）、红细胞沉降率（ESR）、血常规,分别测定各组的阳性率并进行比较。结果急性风湿热组、急性咽炎和扁桃体炎组、急性肾小球肾炎组抗DNA酶B阳性率分别为70.8%、33.3%、83.3%,高于ASO阳性率54.2%、23.3%、60.0%,差异有统计学意义（P〈0.05）；两者联合检测阳性率分别为91.7%、53.3%、93.3%,均高于ASO单独检测阳性率,差异有统计学意义（P〈0.05）。风湿性心脏病活动期抗DNA酶B阳性率为87.5%,明显高于非活动期抗DNA酶B阳性率21.4%,差异有统计学意义（P〈0.05）。结论抗DNA酶B及ASO检测对于小儿链球菌感染病程的不同时期有不同意义,联合检测能明显提高检出阳性率,抗DNA酶B对判断链球菌感染相关疾病活动有一定意义。

Objective To investigate the value of anti-DNA enzyme B on related diseases in children with Streptococ-cus infection. Methods 114 cases of patients with Streptococcus infection in our hospital from January 2012 to October 2013 were collected,and were divided into acute rheumatic fever group,acute pharyngitis and tonsillitis group,acute glomerulonephritis group,rheumatic heart disease group,at the same time,30 cases of healthy children were selected as control group.Anti-DNA enzyme B was detected by micro-neutralization method,Anti-streptolysin O （ASO） was detected by specific protein analyzer immune nephelometry,and pharyngeal swab culture,C-reactive protein （CRP）,erythrocyte sedimentation tate （ESR）,blood routine were detected,at last the positive rate of each group were compared. Results Acute rheumatic fever group,acute pharyngitis and tonsillitis group,acute glomerulonephritis group of anti-DNA enzyme B positive rate was 70.8%,33.3%,83.3% respectively,higher than 54.2%,23.3%,60.0% of ASO positive rate,the difference was significant （P〈0.05）;the positive rate of combined detection was 91.7%,53.3%,93.3%,higher than the positive rate of ASO detection alone,the difference was significant （P〈0.05）.Active rheumatic heart disease of anti-DNA enzyme B positive detection rate was 87.5%,higher than 21.4% of unactive rheumatic heart disease,the difference was significant （P〈0.05）. Conclusion The detection of anti-DNA enzyme B and ASO have different meanings for different stages of children with Streptococcus infection,joint detection of two methods can improve the positive rate,anti-DNA enzyme B has certain significance to the activities related to judge the Streptococcus infection disease.