Bim蛋白、端粒酶蛋白在骨恶性纤维组织细胞瘤中的表达及临床意义

Expressions and Clinical Significances of Bim Protein and Telomerase Protein in Malignant Fibrous Histiocytoma of Bone

目的探讨淋巴细胞瘤-2(Bcl-2)相互作用的细胞凋亡调节因子(bcl-2 interacting mediator of cell death,Bim)蛋白、端粒酶蛋白在骨恶性纤维组织细胞瘤中的表达及临床意义。方法采用免疫组化(EnVisionTM)法分别检测40例骨恶性纤维组织细胞瘤组织(观察组)及40例骨恶性纤维组织细胞瘤旁组织(对照组)中Bim、端粒酶蛋白的表达情况。结果 Bim蛋白、端粒酶蛋白在观察组、对照组阳性率的差异均有统计学意义(χ2=8.71,P<0.01;χ2=22.17,P<0.01)。Bim蛋白、端粒酶蛋白的表达均与骨恶性纤维组织细胞瘤直径、临床分期有关(P<0.05),而与患者年龄、性别、肿瘤部位及组织学类型无关(P>0.05)。Bim蛋白与端粒酶蛋白在骨恶性纤维组织细胞瘤组织中的表达呈显著负相关(r=-0.56,P<0.01)。结论 Bim蛋白和端粒酶蛋白均参与了骨恶性纤维组织细胞瘤的形成,同时检测这2项指标有利于判断肿瘤恶性程度及预后。

Objective To discuss the expressions and clinical significances of Bim （ Bcl-2 interacting mediator of cell death） protein and telomerase protein in malignant fibrous histiocytoma of bone. Methods The expressions of Bim protein and telomerase protein were respectively detected by immunohistochemisty （ EnvisionTM ） in tissues of 40 pa-tients with malignant fibrous histiocytoma of bone （ observational group） and 40 patients＇ surrounding tissues with malig-nant fibrous histiocytoma （ control group） . Results The differences in positive rate of Bim and telomerase protein be-tween observation group and control group were statistically significant （χ2 =8. 71, P〈0. 01; χ2 =22. 17, P〈0. 01）. The expressions of Bim and telomerase protein were all associated with tumor diameter and clinical stages （ P〈0. 05 ） , but the expressions was not associated with age, gender, tumor position and histological type （P〉0. 05）. There was ob-viously negative correlation between expressions of Bim and telomerase protein （r= -0. 56, P〈0. 01） in malignant fi-brous histiocytoma of bone. Conclusion Bim and telomerase protein are involved in the development of malignant fi-brous histiocytoma of bone. Defection of the two expressions at the same time is helpful in judging tumor malignancy level and prognosis.