DTPA和钐对小鼠肝组织的影响

Effects of DTPA and Sm on the liver of mice

将昆明小鼠随机分为生理盐水组、二乙基三胺五乙酸(DTPA)组、SmCl3暴露组、DTPA+钐暴露组,分别于处理1 d、7 d、14d时处死解剖,计算肝脏器系数、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性及测定肝匀浆中的钐质量比,研究DTPA和钐对小鼠肝组织的影响。结果表明,DTPA可引起肝细胞肿胀、SOD活性升高(p<0.01),但引起的MDA含量与肝脏器系数变化与生理盐水对照组相比无显著差异。DTPA促排钐后小鼠脏器系数明显低于生理盐水对照组(p<0.01);处理1 d后SOD活性低于生理盐水对照组(p<0.05),7 d后SOD活性高于生理盐水对照组(p<0.01);MDA含量仅7 d时明显低于生理盐水对照组(p<0.01);肝匀浆组织中钐质量比较钐暴露组低(p<0.01),到14 d时钐质量比为8.590 0μg/g,与生理盐水组无明显差异。可见,DTPA对小鼠肝脏组织病理有影响,体内促排钐效果明显,可减少钐对肝组织的损伤。

To investigate the effects of DTPA and samarium on the liver tissues of mice, we have divided Kunming mice randomly into four groups （30 mice in each group）, that is, the physiological saline control group, the DTPA （ 0.7 mmol/kg） group, the SmCl3 （ 400 mg/kg） group and 400 mg/kg SmCl3 ＋ DTPA. SmCl3 and DTPA were given separately through peritoneal injection and intravenous in- jection with a single dose. The said mice were killed then at the 1 st, 7th and 14th day separately. And, then, the organ coefficient of liver, the activities of superoxide dismutase （SOD）, the contents of malondialdehyde （MDA） and Sm in the liver were also detected. The results of the experimental group treated by DTPA when compared with the physiological saline control group indicate that the liver cells swelled up noticeably, with the SOD activity significantly increased （p 〈 0.01 ） but with no change in MDA content and organ coefficient of the liver. The organ coefficients of liver of SmCl3 ＋ DTPA group turned to be significantly lowered （p 〈 0.01 ） and showed an increasing trend, the SOD activity was becoming significantly lower （p 〈 0.05） on the 1st day and then becoming noticeably higher （p 〈 0.05） on 7th day later. In addition, the MDA content was merely appeared noticeable difference and lower significantly （p 〈 0.01 ） on the 7th day when compared with the physiological saline control group and DTPA group. At the same time, the mass ratio of samarium has been found reduced to 8.590 0 μg/g in SmCl3 ＋ DTPA group, and no noticeable difference was found with the physiological saline control group on the 14th day, which is significantly lower than that in SmCl3 group （p 〈 0.01 ）. All this indicates that DTPA has a certain effect on the liver histopathology of the mice such as the cellular swelling of the liver tissue, etc. However, the damage of the liver histopathology of the said mice turned to be abated when DTPA acted as eccritic forsamarium.