葛根素对早期2型糖尿病大鼠糖脂代谢及胃动力的影响

Effects of Puerarin on Glucose, Lipid Metabolism and Gastric Motility in Early Period Type 2 Diabetic Rats

目的观察葛根素对早期2型糖尿病(T2DM)大鼠糖脂代谢及胃动力的影响,探讨其可能的作用机制。方法高脂高糖饲料加多次注射小剂量链脲佐菌素制备T2DM大鼠模型,随机分为正常对照组、正常干预组、T2DM对照组、T2DM干预组,正常干预组和T2DM干预组给予葛根素400 mg/(kg?d)灌胃,正常对照组和T2DM对照组每日灌胃等量PBS缓冲液。药物干预5周。核素法测定各组大鼠胃半排时间、排空速率,测定空腹血糖(FBG)、糖化血清蛋白(GSP)、游离脂肪酸(FFA)、总胆固醇(TC)、三酰甘油(TG)和胰岛素(INS)的含量。结果与正常对照组比较,T2DM对照组FBG、GSP、FFA、TC和TG水平升高,INS下降,胃半排时间缩短、排空速率加快,差异有统计学意义(P<0.05,P<0.01);与T2DM对照组比较,T2DM干预组TG、GSP和FFA浓度降低,胃半排时间延长、排空速率减慢,差异有统计学意义(P<0.05)。多因素逐步回归分析显示,FBG与胃半排时间相关。结论葛根素可以改善早期T2DM大鼠的TG、GSP、FFA及胃动力异常。

Objective To investigate the effects of Puerarin on glucose and lipid metabolism and gastric motility in early period type 2 diabetic （T2DM） rats. Methods Rat model of T2DM was established by high fat-sugar diet fed and low-dose streptozotocin-treated. SD rats were divided randomly into normal control group （NC）, normal＋Puerarin group （NP）, diabetes control group （DC） and diabetes＋Puerarin group （DP）. NP and DP rats were given Puerarin 400 mg/（kg.d） once per day for 5 weeks, NC and DC rats were given PBS. Half time of gastric emptying and emptying rate were evaluated by SPECT. The serum level of FBG, GSP, FFA, TC, TG and INS were measured by kit. Results Compared with NC group, DC rats had higher FBG, FFA, TC, GSP, TG and emptying rate, but INS and half time of gastric emptying decreased significantly （P〈0.05, P〈0.01）. Compared with DC group, TG, GSP, FFA and emptying rate of DP rats were reduced （P〈0.05）, but had more half time of gastric emptying （P〈0.05）. The results of multivariate stepwise regression analysis showed that FBG related to half time of gastric emptying. Conclusion Type 2 diabetic rats have faster gastric motility, higher blood glucose and lipid. Puerarin might improve the disorders of GSP, TG, FFA and gastric emptying in diabetic rats.