两极隔离式双极电极的电化学发光行为及其分析特性研究

Electrochemiluminescence Behavior of Luminol at Closed Bipolar Electrode and Its Analytical Application

设计了一种两极隔离式双极电极电化学发光装置,并以此装置为基础,研究了电化学发光试剂鲁米诺在此装置中的电化学发光行为和分析特性。通过外加电场的诱导,可以在双极电极的两极富集、分离对应的阴、阳离子,使鲁米诺阴离子富集于双极电极的阳极端,待检测的阳离子富集于双极电极阴极端。根据鲁米诺在阳极端的电化学发光信号定量分析阴极端所富集的阳离子的总量。在最佳条件下,鲁米诺电化学发光信号与分析物浓度在1.0×10^-9~1.0×10^-8mol/L范围内呈线性关系,检出限达1.1×10^-10mol/L。以此为基础,发展了一种电化学发光方法测定溶液电导的新方法。

A new closed bipolar electrode electrochemiluminescence （ ECL）-based device was designed, and further used to investigate the ECL behaviors of luminol in this device. Our results showed that, while a suitable voltage was applied to the two poles of the closed bipolar electrode, both the positively charged ions and luminol-based anionic ions could be enriched on the two poles of the closed bipolar electrode, respectively. More importantly, the ECL signals, generated from the electro-oxidation of luminol on anodic pole, were found to be related to the total amount of positively charged ions on the cathodic pole of the closed bipolar electrode. Under the optimum experimental conditions, the ECL response was linearly to the concentration of analyte in the range of 1. 0×10^-9-1. 0×10^-8 mol/L with a detecting limit of 1. 1×10^-10 mol/L. Based on this finding, a new ECL method for sensing the solution conductance was developed.