循环酶法与荧光偏振免疫法测定血清同型半胱氨酸水平的结果比较

Comparing Circulation Enzymatic and Fluorescence Polarization Immunoassay Method for Serum Homocysteine Levels

目的:比较循环酶法和荧光偏振免疫法在血清同型半胱氨酸水平测定中的结果,探讨快速简便检测同型半胱氨酸的方法。方法:选取糖尿病、高血压、冠心病患者以及正常健康志愿者各20例为观察对象,同时应用循环酶法和荧光偏振免疫法对所有患者的血清标本进行检测,比较两种方法测得的血清同型半胱氨酸水平。结果:循环酶方法检测血清同型半胱氨酸的低值血清批内CV为3.06%,中值血清批内CV为3.97%,高值血清批内CV为4.01%;低值血清批间CV为3.11%,中值血清批间CV为3.92%,高值血清批间CV为4.05%;回收率为102.01%;线性回归方程为Y=0.98X+0.06,r2=0.98;血氨浓度<70μmoL/L时,同型半胱氨酸浓度测定值与原始值的偏差均<5%。经循环酶法检测,糖尿病组、高血压组、冠心病组和健康组观察对象血清同型半胱氨酸浓度分别为8.87±1.21(μmoL/L)、10.21±1.02(μmoL/L)、14.52±1.31和8.11±0.14(μmoL/L),比荧光偏振免疫法测得的结果无明显差别,比较差异均无统计学意义(P>0.05)。结论:循环酶法利用全自动生化分析仪测定血清同型半胱氨酸具有较好的精密度和回收率,可获得可靠准确的结果,且成本较低,操作较简便。

To compare the results of circulating enzymatic and fluorescence polarization im-munoassay method for serum homocysteine levels , and discuss the quick and easy detection of homocysteine . Method：20 patients with diabetes , high blood pressure , coronary heart disease and 20 cases normal healthy volunteers were selected as the observation object , serum specimens were detected by circulating enzymatic and fluorescence polarization immunoassay method, and then serum homocysteine levels was compared .Re-sult：By the circulation enzyme method to detect serum homocysteine , in low serum batch CV was 3.06%, the median serum group within the CV was 3.97%, within the high value of serum CV was 4.01%;Low CV between serum group was 3.11%, the median CV between serum group was 3.92%, the high value of serum between CV was 4.05%;The recovery rate was 102.01%;Linear regression equation was Y =0.98X＋0.06, r2=0.98;When blood ammonia concentration was less than 70μmoL/L, the deviation of the homocysteine concentrations of the original value and the measured value was less 5%.By circulating enzymatic detection of diabetes, hypertension , coronary heart disease group and healthy group , serum homocysteine concentra-tions were 8.87 ±1.21 （μmoL/L）, 10.21 ±1.02 （μmoL/L）, 14.52 ±1.31（μmoL/L） and 1.31 ±0.14 （μmoL/L） , which were no significant difference with the fluorescence polarization immunoassay measured results, the comparing differences were no statistical significance （P 〉0.05）.Conclusion：The circulating enzymatic automatic biochemical analyzer has been applied to the determination of serum homocysteine with good precision and recovery , and reliable and accurate results can be obtained , and the cost is lower , the operation is simpler .