真菌激发子诱导油菜抗菌核病研究

Induced Resistance of Brassica napus by Fungal Elicitors to Sclerotinia sclerotiorum

为了提高油菜对菌核病的抗性,利用病原菌激发子喷施油菜植株,分析其防御体系中酚类物质的变化及对病原菌入侵的抗性。结果表明,多糖激发子和细胞壁激发子均能迅速激活油菜的免疫机制,促进酚类物质的合成,多糖激发子诱导后96h多酚含量达到最大值(0.229mg/g),细胞壁激发子诱导120h后多酚含量达到最大值(0.267mg/g),且提取的植物多酚在体外培养条件下对油菜菌核病菌的菌丝生长有显著的抑制作用,特别是细胞壁激发子诱导组的提取物抑菌率可达60.70%。这说明病原菌激发子可以用于油菜菌核病的防治。

In order to enhance the resistance of Brassica napus to Sclerotinia sclerotiorum,the experiments investigated the effects of fungal elicitors,such as cell wall fragment and polysaccharose on Brassica napus.The results showed that the two elicitors both could activate the immune mechanism of Brassica napus,and enhance the production of polyphenol.The production of polyphenol reached the maximum,0.267 mg/g and 0.229 mg/g,respectively induced by the cell wall elicitor after 120 hand by polysaccharose elicitor after 96 h.The extracts of polyphenol from Brassica napus showed strong inhibition on the mycelia growth in vitro,especially the extracts from the treatment by cell wall elicitor had inhibition rate of 60.70%.These results indicate that fungal elicitors can be used for the control of Sclerotinia sclerotiorum.