SF/COL/PLLA静电纺丝三维纳米纤维支架材料的性能研究

A research on the performance of SF/COL/PLLA electrospun three-dimensiona nanofiber scaffold

基于PLLA(左旋聚乳酸)的优异性能,以六氟异丙醇(HFIP)为溶剂采用静电纺丝法制备质量比为0∶100,30∶70,50∶50,70∶30和100∶0的8%SF/COL/PLLA共混纳米纤维支架,并用浓度为25%戊二醛蒸汽交联48h,通过扫描电镜、X射线衍射、热重分析仪、接触角检测仪对共混材料交联前后的理化性能进行表征。结果表明纳米纤维直径均一,分布均匀;交联后SilkI形成稳定的β折叠,结晶度增强,且交联后材料结晶度明显较交联前增强;热稳定性也随着PLLA浓度的增加愈加稳定。交联后的材料接触角均大于90°表明材料为疏水性,PLLA的加入降低了材料的亲水性能。复合人牙周膜干细胞和材料培养,分析材料的生物学性能,结果证明细胞培养5,7和9d时,细胞在不同比例的支架上粘附、铺展和增殖均良好,但细胞与质量比为30∶70的SF/COL/PLCL纤维膜复合生长最好。

The aim of this study was to prepare a kind of nanofiber scaffolds composed of different mass ratios of silkfi-broin（SF）/collagen/PLLA by using electrospinning technique and to evaluate its properties.1 ,1 ,1 ,3 ,3 ,3-hexafluoro-2-propanol （HFIP）was taken as solvent to prepare the nanofiber scaffolds and the mass ratios of SF/collagen/PLLA were set as 0∶100,30∶70,50∶50,70∶30,100∶0.And then the preoperties of the as-prepared samples were tested before and after crosslinked by 25% glutaraldehyde vapor for 48 h.The scaffolds were characterized by scanning electron mi-croscopy （SEM）,X-ray diffraction （XRD）,thermo gravimetric （TG）analysis and the hydrophily was analysed by water contact angle （WCA）analysis.The results of SEM revealed that the uniformity of the blending nanofibers had im-proved.The thermal stability of the scaffold material increased with the increase of the blend ratios of PLLA.The XRD results indicated that stableβ-folding conformation of silk has formed and the crystallization of materials has increased. The contact water angle meter analysis suggested that the hydrophilic property of the blending composite nanofibers de-creased with the existence of PLLA.The periodontal ligament stem cells （PDLSCs）were seeded on the blending com-posite nanofibers in vitro.The cells grew well on the surface of materials,and had great adhesion and proliferation abili-ties.The cytocompatibility and physicochemical properties of the scaffold with content of 70% PLLA are superior to other groups,which suggest the as-prepared sampleis expected to become a new type of scaffold forperiodontal tissue en-gineering.