胸腺五肽表面印迹硅胶的制备及识别性能的研究

Preparation of thymopentin molecularly imprinted polymers on SiO_2 and recognition characteristics

用计算机分子模拟对功能单体和模板分子间的结合作用进行了研究,选择结合能最强的甲基丙烯酸作为功能单体。以γ-(甲基丙烯酰氧)丙基三甲氧基硅烷(MPS)为媒介,将功能单体甲基丙烯酸(MAA)接枝于SiO2微粒表面,制得了功能接枝微粒PMAA/SiO2。以PMAA/SiO2为载体,甲基丙烯酰胺(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,采用溶液聚合法制备了以胸腺五肽为模板分子的表面印迹微粒(Tp5-MIP)。分别通过动态吸附实验和等温吸附实验研究了该印迹微粒对胸腺五肽的识别特性。结果表明,该印迹微粒对印迹分子具有较好的亲和能力及选择性,其识别能力来自于印迹得到的识别位点。

The functional monomer of methylacrylic acid （MAA）and acrylamide （AM）were evaluated using computer simulation technological,the MAA was selected as functional monomer.By coupling action of 3-methacryloxypropyl trimethoxysilane （MPS）,polymethylacrylic acid （PMAA）was grafted on the surface of silica particles.Using solution polymerization method,the molecular imprinted polymer Tp5-MIP was obtained using PMAA/SiO2 as vector,thymopentin as the template molecular and ethylene glycol dimethacrylate （ED-MA）as the crosslinking agent.Both static and dynamic binding properties of Tp5-MIP for thymopentin were studied.Result shows that Tp5-MIP exhibits excellent binding affinity and specific selectivity for the template molecule.The recognition resulted from the imprinting process.