期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

用于肿瘤治疗的siRNA纳米输送系统 被引量：4

siRNA nano-delivery systems for cancer therapy
原文传递
导出
摘要 人类恶性肿瘤的发生发展过程涉及多种基因的表达和功能异常。小干扰RNA(Small interfering RNA,siRNA)通过碱基配对的作用方式干扰特定胞浆内信使RNA(mRNA),从而介导基因沉默,其研究在肿瘤治疗的研究和应用中取得了突出进展。然而,siRNA体内给药仍存在诸多问题,相应的siRNA输送系统呈现出多样化发展趋势。本文总结归纳了肿瘤治疗研究中各种不同的siRNA纳米输送策略,包括提高肿瘤靶向性、增强细胞摄取效率和改善溶酶体逃逸能力等方面,旨在为设计新型siRNA纳米输送系统提供参考。 The onset and progression of human cancer involve the expression and functional abnormalities of a variety of genes. Small interfering RNA （siRNA） mediates gene silencing by way of interference effects of target messenger RNA with base pairing, which has made outstanding progress in research and application in cancer therapy. However, there are many problems for siRNA delivery in vivo. This review summarizes a variety of differ- ent siRNA delivery systems used for cancer treatment and some important strategies for improving siRNA transfection based on targeted accumulation in tumor, enhancing cellular uptake and improving endosomal/lysosomal escape. aiming to provide the reference for the design of siRNA delivery systems.
作者 邱崇 张亚琦 刘卫中 王坚成 张强
机构地区 北京大学药学院药剂学系
出处 《中国新药杂志》 CAS CSCD 北大核心 2014年第16期1885-1892,共8页 Chinese Journal of New Drugs
关键词 肿瘤治疗 SIRNA 纳米输送系统 靶向递送 细胞摄取 溶酶体逃逸 基因沉默 cancer therapy siRNA nano-delivery systems targeted delivery cellular uptake endosomal escape gene silencing
分类号 R94 [医药卫生—药剂学]
  • 相关文献

参考文献64

  • 1DOMINSKA M, DYKXHOORN DM. Dykxhoorn, Breaking down the barriers: siRNA delivery and endosome escape [ J]. J Cell Sci, 2010, 123(Pt 8) :1183 - 1189.
  • 2NGO H, TSCHUDI C, GULL K, et al. Double-stranded RNA induces mRNA degradation in trypanosoma brucei[ J]. Proc Natl Acad Sci USA, 1998,95 (25) : 14687 - 14692.
  • 3SCHERER LJ, ROSSI JJ. Approaches for the sequence-specific knockdown of Mrna[ J]. Nat Biotechnol, 2003, 21 ( 12 ) : 1457 - 1465.
  • 4SAH DW. Therapeutic potential of RNA interference for neuro- logical disorders[ J ]. Life Sci, 2006, 79 ( 19 ) : 1773 - 1780.
  • 5MOREL JB, GODON C, MOURRAIN P, et al. Fertile hypomor- phic ARGONAUTE ( agol ) mutants impaired in post-transcrip- tional gene silencing and virus resistance[ J]. Plant Cell, 2002, 14 ( 3 ) :629 - 639.
  • 6TAVERNARAKIS N, WANG SL, DOROVKOV M, et al. Herit- able and inducible genetic interference by double-stranded RNA encoded by transgenes[ J]. Nat Genet, 2000, 24(2):180-183.
  • 7JACKSON AL, BARTZ SR, SCHELTER J, et al. Expression profiling reveals off-target gene regulation by RNAi[ J]. Nat Bio- technol, 2003, 21 (6) :635 - 637.
  • 8BUMCROT D, MANOHARAN M, KOTELIANSKY V, et al. RNAi therapeutics: a potential new classof pharmaceutical drugs [J~. Nat Chem Biol, 2006, 2(12) :711 -719.
  • 9DYKXHOORN DM, LIEBERMAN J. Knocking down disease with siRNAs[J]. Cell, 2006, 126(2) :231 -235.
  • 10LI H, LI WX, DING SW. Induction and suppression of RNA si- lencing by an animal virus [ J ]. Science, 2002, 296 ( 5571 ) : 1319 - 1321.

二级参考文献83

  • 1GAO Y, CHEN L, GU W, et al. Targeted nanoassembly loaded with docetaxel improves intracellular drug delivery and efficacy in murine breast cancer model[ J ]. Mo Pharmaceutics,2008,5 (6) : 1044-1054.
  • 2YU D H, LU Q, XIE J, et al. Peptide-conjugated biodegradable nanoparticles as a carrier to target paclitaxel to tumor neovasculature [J]. Biomaterials, 2010,31 ( 8 ) : 2278-2292.
  • 3ARAP W, PASQUALINI R, RUOSLAHTI E. Cancer treatment by targeted drug delivery to tumor vasculaturc in a mouse model [J]. Science,1998, 279(5349): 377-380.
  • 4MAJHEN D, GABRILOVAC J, ELOIT M, et al. Disulfide bond formation in NGR fiber-modified adenovirus is essential for retargeting to aminopeptidase N [ J ]. Biochem Biophys Res Commun, 2006,348( 1 ) : 278-287.
  • 5ELLERBY H M, ARAP W, ELLERBY I, M, et al. Anti-cancer activity of targeted pro-apoptotic peptides [ J]. Nat Med, 1999,5 (9) : 1032-1038.
  • 6CURNIS F, SACCHI A, BORGNA L, et al. Enhancement of tumor necrosis factor alpha antitumor immunotherapeutic properties by targeted delivery to aminopeptidase N ( CD13 ) [ J ], Nat Biotechnol,2000, 18( 11 ) : 1185-1190.
  • 7WANG X, WANG Y, CHEN X, et al. NGR-modified micelles enhance their interaction with CD13-overexpressing tumor and endothelial ceils [ J ]. J Controlled Release, 2009, 139 ( 1 ) :56-62.
  • 8NEGUSSIE A H, MILLER J L, REDDY G, et al. Synthesis and in vitro evaluation of cyclic NGR peptide targeted thermally sensitive liposome [ J ]. J Controlled Release, 2010, 143 ( 2 ) : 265- 273.
  • 9MAI J, SONG S, RUI M,et al. A synthetic peptide mediated active targeting of cisplatin liposomes to Tie2 expressing cells [ J ]. J Coatrolled Release, 2009, 139 ( 3 ) : 174-181.
  • 10CHEN X, WANG X, WANG Y, et al. Improved tumor-targeting drug delivery and therapeutic efficacy by cationic liposome modified with truncated bFGF peptide [ J ]. J Controlled Release, 2010, 145(1 ) :17-25.

共引文献1

同被引文献50

引证文献4

二级引证文献4

中国新药杂志
2014年 第16期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部