二氯苯染毒对小鼠组织中Zn、Cu、Fe含量的影响

Effect of Subacute Intoxication of p- DCB on Levels of Zn,Cu,Fe in Tissue of Mice

采用亚急性试验,探讨对二氯苯(p-DCB)对小鼠组织中Zn、Cu、Fe的影响。选用36只小鼠,雌雄各半,随机分成3组,每组12只,分别给予p-DCB O(对照组)、450、900mg·kg-1,每天灌胃染毒1次,连续7 d,染毒结束后24 h处死动物并称体质量、肝、肾质量,计算脏器系数。用火焰原子吸收法测定肝脏、血液和肾脏中Zn、Cu、Fe含量。与对照组相比,各染毒组体质量无明显变化。各组肝体系数无差异,但染毒组肾体系数明显高于对照组(P<O.05)。900 mg·kg-1组肝脏中Zn含量及w(Zn)/w(Cu)值较450 mg·kg-1组有明显升高。与对照组相比,各染毒组血液中Zn含量呈现下降趋势,900 mg·kg-1组较对照组明显下降(P<O.05)。900 mg·kg-1组肾脏中Cu质量分数明显低于450 mg·kg-1组,Fe质量分数则较对照组及450mg·kg-1组均有降低,p-DCB可影响小鼠组织中元素Zn、Cu、Fe的含量及不同器官的再分布,提示微量元素失衡可能是p-DCB毒作用的重要机制。

To study the concentrations of Zn, Cu, Fe in blood. Kidney and liver of mice subacutely exposed to p - Dichlorobenzene （ p - DCB）, to provide a basis in the toxicity mechanism of p - DCB. Thirtysix mice were randomly divided into three groups, oil （solvent control group ）, 450, 900 mg·kg^-1 b.w. of p - DCB （ p - DCB groups） were given respectively by daily gavage for one week, and then the mice were sacrificed. The concentrations of Zn （zinc）, Cu （copper）, Fe （iron） in theliver, kidney and whole blood of mice were determined. The change of the liver/body weight was increased significant, though there was an tendency. Compared with control group, the kidney, body weight of p - DCB groups was not significant （ P 〈 0. 05 ）, the level of Zn and w （Zn）/w （Cu） in the liver of mice in high does group significantly. The levels of Cu in kidney in the high dose group were also decreased significantly compared with low dose group the results show that p - DCB my induce changes in the level of elements in mice, and be implied that imbalance of these trace elements may play a key role in the mechanism of toxicity of p -DCB.