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基因沉默抑制子HC-Pro表达载体的构建

Establishment of expression vector of gene silencing suppressor HC-Pro
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摘要 RNA沉默广泛存在于植物等大多数真核生物中,能够防御外源基因的入侵和调控基因表达等.然而,基因沉默抑制子HC-Pro蛋白的具体功能的研究并不十分清楚.本文重点介绍了基因沉默抑制子HC-Pro的表达载体pBI121-HC-Pro的构建及HC-Pro基因在烟草(Nicotiana benthamiana)中的稳定表达.并利用半定量RT-PCR技术检测了目的基因HC-Pro的表达,检测结果表明我们获得了HC-Pro基因稳定表达的转基因烟草株系,为进一步深入研究基因沉默抑制子HC-Pro的功能奠定了实验基础. RNA silencing is a conserved pathway that results in blockage of gene expression in both the cytoplasm and nucleus of eukaryotic organisms and is involved in regulating gene expression. Action of the gene silencing suppressor HC-Pro protein encoded by TEV is not clear. In this paper,we focuse on establishment of expression vector pBI121-HC-Pro of gene silencing suppressor HC-Pro and stable expression of HC-Pro gene in NIcotIana benthamIana plants. The expression of HC-Pro gene in NIcotIana benthamIana plants was detested by semi-quan-titative RT-PCR. The results showed that transgenetic tabacco lines of HC-Pro gene were acquired,and provided study base for action of the gene silencing suppressor HC-Pro protein.
作者 金太成 许亚男 郑爽 张晓华 冯茹茹 刘秋霞 李双双 杨丽萍
机构地区 吉林师范大学生命科学学院
出处 《吉林师范大学学报（自然科学版）》 2014年第3期135-137,141,共4页 Journal of Jilin Normal University:Natural Science Edition
基金 国家青年科学基金项目(31301043) 吉林省科技发展计划青年科研基金项目(20130522182JH)
关键词 基因沉默抑制子 蚜传辅助因子 稳定表达 烟草 gene silencing suppressor HC-Pro stable expression NIcotIana benthamIana
分类号 Q94 [生物学—植物学]
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参考文献15

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吉林师范大学学报（自然科学版）
2014年 第3期

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