PTEN和Notch1在结直肠癌组织中的表达及临床意义

The expression and clinical significance of PTEN and Notch1 in colorectal cancer

目的:探讨人第10号染色体缺失的磷酸酶及张力蛋白同源物(PTEN)和Notch1在结直肠癌的发生、发展中的作用和机制以及二者的相互关系。方法:采用免疫组织化学法检测60例结直肠癌和30例癌旁非肿瘤性组织PTEN和Notch1表达,分析其与临床病理指标的关系及二者的相关性。结果:结直肠癌组织中PTEN蛋白阳性率为58.3%,明显低于正常结直肠组织的6.7%(P<0.01),且在分化程度、Dukes临床分期、浆膜浸润和淋巴结转移间差异均有统计学意义(P<0.05)。结直肠癌组织中Notch1的阳性率为45.0%,明显高于正常结直肠组织的6.7%(P<0.05),且在分化程度、Dukes临床分期、浆膜浸润和淋巴结转移间差异均有统计学意义(P<0.05)。结直肠癌组织中PTEN与Notch1表达呈负相关(P<0.05)。结论:PTEN与Notch1的表达失调可能与结直肠癌的发生、发展相关,对PTEN和Notch1表达水平的联合检测可为早期发现和临床治疗结直肠癌提供理论依据。

Objective：To detect the roles of PTEN and Notch1 in the occurrence and development of colorectal cancer and their mechanisms,and the correlations.Methods：The expressions of PTEN and Notch1 in 60 cases of colorectal cancer tissues and 30 casess of adjacent non-tumor tissues were detected by immunohistochemistry,and their relationships with clinicopathological parameters were analyzed.Results：The positive rate of PTEN expression in colorectal cancer tissue （58.3 ％） was significantly lower than that in non-tumor tissue （96.7％） （P ＜ 0.01）,the differences of the differentiation degree,clinical Dukes stage,serosal invasion and lymph node metastasis between them were statistically significant （P ＜ 0.05）.The positive rate of Notch1 expression in colorectal cancer tissue （45.0％） was significantly higher than that in non-tumor tissue （6.7％） （P ＜ 0.05）,the differences of the differentiation degree,clinical Dukes stage,serosal invasion and lymph node metastasis between them were statistically significant （P ＜ 0.05）.The PTEN expression was negatively correlated to the Notch1 expression in colorectal cancer tissue（P ＜ 0.05）.Conclusions：The dysregulation of PTEN and Notchl expressions may be associated with the occurrence and development of colorectal cancer.The expression levels of PTEN and Notch1 can provide the theoretical basis in the early diagnosis and treatment of colorectal cancer.