microRNA let-7a对乙型肝炎病毒复制的影响

Effect of microRNA let-7a on replication of HBV

目的研究microRNA let-7a(以下简称let-7a)对乙型肝炎病毒(HBV)复制的影响。方法 (1)收集23例伴有慢性活动性乙型肝炎的肝细胞癌(HCC)患者的手术标本,用实时荧光定量PCR(real-time qRT-PCR)检测let-7a在HCC组织中的表达,分析let-7a的表达和HBV复制状态之间的关系。(2)用real-time qRT-PCR法检测HBV稳定复制的HCC细胞系HepG2.2.15及其亲本HCC细胞HepG2的let-7a表达。(3)通过细胞转染,利用let-7a特异的反义寡核苷酸抑制物下调HepG2.2.15细胞中let-7a的表达,通过实时PCR(real-time PCR)检测HBV DNA水平的变化。结果 let-7a在HBV高复制的临床HCC标本中明显高于HBV低复制者(P<0.05)。与HepG2相比,let-7a在HBV稳定复制的HepG2.2.15细胞系中表达上调(P<0.05)。let-7a抑制剂能显著抑制HBV DNA的水平,并且具有时间依赖性(P<0.05)。结论 let-7a的表达和HBV的复制状态呈正相关,let-7a能促进HBV复制。

Objective To investigate the effect of microRNA let-7a on replication of HBV. Methods （1） To detect let-7a with real-time qRT-PCR in 23 liver cancer samples with active chronic hepatitis B, and explore the relationship between let-7a level and HCV replication. （2） To detect let-7a with real-time qRT-PCR in HepG2.2. 15 and HepG2. （3） The specific antisense inhibitor for lev7a was transfected into HepG2.2. 15 cells to down-regulate its expression, and detect HBV DNA with real-time PCR. Results The level of let-7a was significantly higher in liver cancer samples in HBV high replicated rate than that in HBV low replicated rate （P〈0. 05）. The level of let-7a was significantly higher in HepG2.2.15 than that in HepG2 （P〈0.05）. The specific antisense inhibitor for let-7a inhibited HBV replication on HBV DNA levels （P〈0.05）. Conclusions The expression of let-7a has a positive relationship with the replication of HBV. Then, let-7a can promote HBV replication.