贵州和浙江产吴茱萸指纹图谱分析及含量测定

Fingerprint and Content Determination of Euodiae Fructus Collected in Guizhou and Zhejiang

目的:应用UPLC-UV指纹图谱方法,结合吴茱萸中有效成分柠檬苦素、吴茱萸碱和吴茱萸次碱的含量测定,评价不同品种及产地的吴茱萸药材质量,为控制市场上吴茱萸药材质量提供依据。方法:采用ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm),流动相乙腈-水梯度洗脱,流速0.5 mL·min-1,检测波长210 nm,柱温25℃。收集了24批不同产地不同种吴茱萸样品进行指纹图谱分析,结合主成分分析及有效成分含量测定,对吴茱萸药材整体质量进行评价。结果:建立了吴茱萸UPLC-UV指纹图谱,标定了9个共有峰。指纹图谱相似度评价结果显示贵州产吴茱萸质量较稳定、统一,浙江产吴茱萸质量稍不统一。主成分分析结果表明,吴茱萸药材不同种之间成分上存在差异性。产地对吴茱萸药材中柠檬苦素和生物碱含量有影响。结论:指纹图谱结合有效成分含量测定的方法可用于吴茱萸药材的整体质量评价,对吴茱萸药材的品种鉴定及质量控制具有指导意义。

Objective： This study was performed to evaluate the quality of Euodiae Fructus from different varieties and locations and to provide the foundation for quality control it in the market by UPLC-UV fingerprint and content determination of active compounds of limonin,evodiamine and rutaecarpine. Method： The separation was performed on an ACQUITY UPLC HSS T3 column（2. 1 mm × 100 mm,1. 8 μm） with gradient elution using acetonitrile-water asmobile phase. The flow rate was 0. 5 mL·min^-1. UV detecting wavelength was set at 210 nm,and column temperature was set at 25 ℃. The holistic quality of 24 samples were evaluated by fingerprint combined with unsupervised principal component analysis（PCA）. The three active compounds in 24 samples collected from different varieties and locations were determined. Result： An ultra-performance liquid chromatographic method was established for fingerprint analysis,and nine peaks in UPLC profile were found as the same. The result of fingerprint similarity evaluation showed that the quality of Euodiae Fructus collected from Guizhou was stable and consistent,however,that from Zhejiang was inconsistent slightly. The result of PCA showed that there is difference in the components of Euodiae Fructus from different varieties. The contents of limonin, evodiamine and rutaecarpine in Euodiae Frutus were influenced by locations. Conclusion： The fingerprint analysis combined with the content determination of active compounds was available for holistic quality evaluation of Euodiae Fructus,which is directive is directive significance to origin identification and quality control of Euodiae Fructus.