5种小檗属药用植物总生物碱及小檗碱的含量测定

Quantitative Determination of Total Alkaloids and Berberine in Five Medicinal Berberis Plants

目的：对5种小檗属植物（拟豪猪刺、川鄂小檗、芒齿小檗、湖北小檗、汉源小檗,均为药材三颗针来源）根和茎中活性物质总生物碱和小檗碱进行研究,比较5种植物根和茎的总生物碱和小檗碱的含量,为三颗针药材的资源与质量评价提供参考。方法：分别通过酸性染料比色法和高效液相色谱法进行总生物碱及小檗碱含量测定研究。结果：5种植物根和茎的总生物碱含量范围分别为1.60%～4.72%和0.76%～2.70%,小檗碱的含量范围分别为0.70%～2.92%和0.23%～1.07%。湖北小檗、汉源小檗和拟豪猪刺根的总生物碱及小檗碱的含量均较高,是优质的三颗针药材来源,其茎中的总生物碱和小檗碱的含量也较高。结论：所建立的总生物碱和小檗碱的含量测定方法准确、可行,为该药材的质量评价提供了可靠方法。

Objective： The major activity ingredients of Berberis plants are alkaloids. To compare the total alkaloids and individual alkaloid（berberine） contents in roots and stems from five origins of Berberis plants（Berberis soulieana Schneid.,B. henryana Schneid.,B. triacanthophora Fedde,B. gagnepainii Schneid. and B. bergmanniae Schneid.）,whose roots are widely used as a traditional Chinese medicine called ＂Sankezhen＂,and provide some references for resource and quality evaluation of the medicine. Method： Acid dye colorimetry and high performance liquid chromatography（HPLC） were used to measure contents of the total alkaloids and berberine,respectively. Result：Contents for the total alkaloids in root and stem samples were in the range of 1. 60% to 4. 72% and 0. 76% to 2. 70%,while those of the berberine were 0. 70% to 2. 92% and 0. 23% to 1. 07%. With higher contents of the total alkaloids and berberine,the roots of B. soulieana Schneid.,B. gagnepainii Schneid. and B. bergmanniae Schneid. are good sources of Sankezhen,meanwhile the contents were also high in stems of the three plants. Conclusion： The methods established for determination of total alkaloids and berberine were simple,rapid and reliable. Furthermore,the present study provided valuable research methods for the quality evaluation of the plants.