银杏二萜内酯葡胺注射液对大鼠急性脑缺血再灌注损伤的影响

Protective Effects of Ginkgo Terpene Lactones Meglumine Injection on Focal Cerebral Ischemia Reperfusion Injury in Rats

目的:探讨银杏二萜内酯葡胺注射液对线栓法致大鼠急性脑缺血再灌注损伤(MCAO)的保护作用,并探讨其可能的作用机制。方法:雄性SD大鼠96只,随机分为假手术组、模型组、尼莫地平(0.4 mg·kg-1)组、银杏二萜内酯葡胺注射液高、中、低(4.8,2.4,1.2 g·kg-1)剂量组。所有动物手术前2 d尾静脉注射给药(模型组及假手术组给予生理盐水)。除假手术组,其他动物通过右侧大脑中动脉线栓塞法(MCAO)制作局灶性脑缺血再灌注模型。缺血3 h后,所用动物再次尾静脉注射给药。采用脑组织TTC染色法观察梗死率;放免法测定血清中磷酸肌酸激酶脑型同工酶(CK-BB)、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;免疫组化法测定脑组织中半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)表达。结果:与正常组比较,模型组大鼠脑梗死率显著增加,神经缺损症状严重;血清中SOD活性降低,MDA,CK-BB含量显著增加;脑组织中Caspase-3表达量显著升高。与模型组比较银杏二萜内酯葡胺注射液中剂量组能显著减少脑梗死率,改善脑神经功能缺损症状;提高SOD活性,减少血清中MDA,CK-BB含量,抑制脑组织中Caspase-3表达。结论:银杏二萜内酯葡胺注射液对大鼠局灶性脑缺血再灌注损伤具有保护作用,作用机制可能与其修复血脑屏障、降低血清CK-BB含量、抗自由基损伤及抑制Caspase-3表达有关。

Objective： To investigate the protective effects of Ginkgo terpene lactones meglumine injection（GTLMI） on cerebral focal ischemia reperfusion injury induced by middle cerebral artery occlusion（MCAO） in rats. Method： Ninety six male SD rats were divided into six groups,such as sham,model,nimodipine（0. 4 mg ·kg^-1）,and GTLMI（4.8,2.4,1.2 g ·kg^-1） groups. All of rats were given corresponding drugs by tail vein injection before modeling. Except the sham group,the cerebral ischemic stroke model was used. The effects of GTLMI on the brain neurological deficit score,and the brain infraction areas percentage were observed. The influence of GTLMI on the activities of superoxide dismutase（SOD）,content of malondialdehyde（MDA）,creatine kinaes BB isozyme（CK-BB） in blood serum and the activities of Caspase-3 in brain were assayed. Result： Compared with MCAO group,GTLMI could obviously reduce the infract area,increase the activities of SOD,and decrease the content of MDA,CK-BB in blood serum and Caspase-3 in brain.Conclusion： GTLMI can protect the focal cerebral ischemia reperfusion injury in rats. The mechanisms may be related to recovering the blood brain barrier,decreasing the content of CK-BB,scavenging free radicals and reduction of the expression of Caspase-3.