模拟失重大鼠的抑郁样行为学表现

Depressive Behavioral on Rats of Simulated Weightlessness

目的:研究大鼠尾部悬吊模拟失重所致的抑郁样行为学表现并进行初步的机制探讨。方法:利用大鼠模拟失重尾吊14 d,以空场实验、新奇事物实验、新奇抑制摄食实验、强迫游泳和穿梭实验研究大鼠行为学表现,采用酶联免疫分析大鼠血清皮质酮和促肾上腺皮质激素的含量;采用蛋白质印迹法检测大鼠大脑皮层脑源性神经营养因子(BDNF)蛋白的变化。结果:空场试验结果显示,模型组与对照组相比,自主活动能力下降(P<0.05或P<0.01)、爬壁次数减少[模型组(0.70±0.95)次,正常组(10.16±3.78)次,P<0.01],新奇事物和新奇抑制摄食实验潜伏期延长,强迫游泳的不动时间增加,穿梭实验主动穿梭次数减少(P<0.05或P<0.01),血清皮质酮、促肾上腺皮质激素水平升高(P<0.05或P<0.01),大脑皮层中BDNF表达下降(P<0.01)。结论:大鼠尾部悬吊14 d模拟失重可出现抑郁样行为表现。

Objective： To study the depressive behavior induced by simulated weightlessness in rats and the possible mechanism. Method： Simulating weightlessness（SW） 14 days to rats,the open field test,the novel object test,the novelty suppressed feeding test,the forced swimming test and the shuttle box test were applied to evaluate the depressive-behavior in rats. The concentration of serum corticosterone and adrenocorticotropic were measured by enzyme-linked immunosorbent assay（ELISA）,and the changes of the brain-derived neurotrophic factor was measured by western blotting experiment. Result： The results of the open field test were as follows：compared with the control group,the ability of locomotor activity and the number of climbing wall decreased in the SW model rats. The latency of novel object test and the novelty suppressed feeding test in the rats of the model group were longer than those of the control group. The immobility time in the forced swimming test was longer and the number of active avoidance of shuttle box test were lower than the control group. Moreover,the hormone levels of serum corticesterone（CORT） and adrenocorticotropic hormone（ACTH） increased in the rats of the model group than those of the control group. While,the expression of brain-derived neurotrophic factor brain-derived neurotrophic factor（BDNF） in the brain of rats in the model group declined than that of the control group.Conclusion： To simulate weightlessness for 14 days can induce the depressive behavior in rats.