消渴胶囊对2型糖尿病模型大鼠血糖和血脂的作用

Hypoglycemic and Hypolipidemic Effects of Xiaoke Capsules on Type 2 Diabetic Rats

目的:观察消渴胶囊对2型糖尿病模型大鼠的降血糖和降血脂作用。方法:Wistar大鼠70只,其中8只作为正常对照组。其余62只采用高脂高糖饲料喂养加小剂量ip链尿佐菌素(STZ)35 mg·kg-1造大鼠2型糖尿病模型,将血糖值>7.0 mmol·L-1的大鼠按照血糖浓度随机区组分为模型组,消渴胶囊高、中、低剂量组(0.4,0.2,0.1 g·kg-1),阳性药组(格列本脲0.25 mg·kg-1),连续ig给药36 d。给药期间每周测大鼠体质量,测量大鼠给药前1 d及给药后第8,16,24,32 d空腹血糖浓度,末次给药后12 h麻醉后断头处死大鼠。取血清测大鼠的血清胰岛素(INS)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)含量;取大鼠的胰腺组织做组织病理学检查。结果:与正常组比较,模型组大鼠的血糖明显升高(P<0.01),血清INS水平明显降低(P<0.01),血清TC,TG,LDL-C水平明显升高(P<0.01);与模型组比较,消渴胶囊低、中、高剂量组均有明显的降糖作用(P<0.05),消渴胶囊中剂量组可明显降低血清INS,TC,TG和LDL-C的水平(P<0.05)。病理检查结果显示中剂量和高剂量组大鼠胰岛数及岛内细胞数较模型组为多,形态较为规则,胰岛内分泌细胞形态接近正常组。结论:消渴胶囊对2型糖尿病模型大鼠有降血糖以及降血脂的作用。

Objective： To observe hypoglycemic and hypolipidemic effects of Xiaoke capsules on type 2diabetic rats. Method： Except eight Wistar rats were as the normal control,the rest sixty-two type 2 diabetic rats were induced by feeding with high-fat and high-suger diet and intraperitoneal injection of low-dose streptozotocin（STZ,35 mg·kg^-1）. According to the values of blood glucose concentration（7.0 mmol·L^-1）,the rats were randomly divided into diabetic model group,three Xiaoke capsules groups of high dose（0. 4 g·kg^-1）,medium dose（0. 2 g·kg^-1） and low dose（0.1 g·kg^-1）,and positive control（glibenclamide,0.25 mg·kg^-1） group.During 36 d of continuous administration,body weights of the rats were measured weekly. The values of fasting blood glucose concentration were measured on 0, 8, 16, 24, 32 d of the experiment. After the last administration,12 h latter,the rats were decapitated after anesthesia. Blood of the rats were collected for the analysis of serum insulin（INS）,total cholesterol（TC）,triglycerides（TG） and low-density lipoprotein（LDLC） levels. Pancreas of the rats were collected for histopathological examination. Result： Compared with normal group,model group,blood glucose was significantly increased（P〈0. 01）,serum INS was significantly decreased（P〈0. 01）,TC,TG,LDL-C was significantly increased（P〈0.01）. Compared with model group,Xiaoke capsules showed significant hypoglycemic effect（P〈0. 05）,and significant difference was observed compared to diabetic model group on serum INS,TC,TG and LDL-C levels（P〈0. 05）. Histopathological results indicated that more numbers and much more regular shape of islets and islet cells in the medium dose and high dose of treatment groups were observed with comparison to the model group,and the morphology of endocrine cells in islets were close to those of the normal group. Conclusion： Xiaoke capsules can decrease blood glucose,regulate blood lipids in type 2 diabetic rats.