红花夹竹桃寄生中芦丁和广寄生苷含量的高效液相色谱法测定

HPLC determination of rutin and guajeverin in Scurrula parasitica L. parasitizing on Nerium indicum Mill

目的建立HPLC法测定红花夹竹桃寄生中芦丁和广寄生苷含量的方法。方法采用HPLC法，以芦丁和广寄生苷为对照品，色谱柱为依利特Hypersil ODS柱（4．6mm×250mm，5μm），以乙腈－１％乙酸溶液梯度洗脱为流动相，流速为1．Om1·min^-1，检测波长365nm，测定红花夹竹桃寄生中芦丁和广寄生苷含量。结果芦丁、广寄生苷的线性范围均为8．24—329．6Iμg·ml^-1，相关系数分别为0．9995，0．9996，加样回收率（n=6）分别为101．76％，103．47％，RSD分别为1．34％、0．76％，红花夹竹桃寄生中芦丁和广寄生苷的平均含量分别为0．32％，0．11％。结论该方法准确、重现性好，可作为红花夹竹桃寄生药材的质量控制标准。

Objective To establish an HPLC method for the determination of the contents of rutin and guajeverin in Nerium indi-cure Mill. Methods Contents of rutin, guajeverin in Nerium indicum Mill was measured by HPLC a column of Hypersil ODS （4. 6mm×250mm,5μm）,with mobile phase consisted of acetonitrile（B） -1% acetic acid（A） with the gradient elution,flow rate： 1.0ml· min^-1, detection wavelength of 365nm. Results The calibration curves showed good linearity in the range of 8.24 -329. 6 μg·mL^-1（ rutin, guajeverin ） ; correlation coefficients were 0. 9995,0. 9996. The average recoveries （ n = 6 ） were 101.76 %, 103.47% ;RSD were 1.34% ,0.76% respectively. The average contents（ n = 5 ） were 0.32% （rutin） ,0.11% （ guajeverin）. Conclusion An accurate and reproducible method can be used for the quality control of Scurrula parasitica L. parasitizing on Nerium indicum Mill.