程序性细胞死亡因子5在子宫内膜癌组织中的表达及意义

Expression of programmed cell death 5 in endometrial adenocarcinoma and its clinical significance

目的：探讨程序性细胞死亡因子5（PDCD5）的表达与子宫内膜癌发生的相关性。方法收集行手术治疗的60例子宫内膜癌组织、40例正常增生期子宫内膜组织及28例子宫内膜不典型增生组织，实时荧光定量PCR检测PDCD5 mRNA的表达，免疫组织化学法检测蛋白的表达。结果在正常增生期子宫内膜、子宫内膜不典型增生、子宫内膜癌组织中，PDCD5 mRNA的相对定量依次降低[（1．21±0．16）、（0．83±0.07）、（0．42±0．02），F＝5．637，P＜0．05]；PDCD5蛋白的阳性表达率依次降低（82．5％、64．3％、45．0％，χ2＝27．663，P＜0．05）；PDCD5的表达水平与子宫内膜癌患者的临床分期、组织学分级、肌层浸润以及淋巴结转移有关（χ2＝20．875、11．915、9．409、11．148，均P＜0．05），而与年龄无关（χ2＝0．287，P＞0．05）。结论PDCD5的表达下调与子宫内膜癌的发生、发展有关，有可能成为一种潜在的早期预测子宫内膜癌的标志物。

Objective This study was designed to investigate the relationship between programmed cell death 5 （PDCD5） and endometrial carcinoma .Methods 60 tissue samples of endometrial carcinomas ,40 normal proliferative endometrium tissues and 28 endometrial atypical hyperplasia tissues were obtained from patients who underwent operation.The expression of PDCD5 was detected by Real-time PCR and immunohistochemical analysis . Results In normal proliferative endometrium , atypical hyperplasia tissues and endometrial carcinoma , the PDCD5 mRNA was gradually decreased[（1.21 ±0.16）,（0.83 ±0.07）,（0.42 ±0.02）,F=5.637,P〈0.05];The posi-tive rate of protein was gradually decreased （82.5%,64.3%,45.0%,χ2 =27.663,P〈0.05）;The expression of PDCD5 was correlated with TNM stage , histological grade , depth of myometrial invasion and lymphatic metastasis （χ2 =20.875,11.915,9.409,11.148,all P〈0.05）,but was not correlated with ages （χ2 =0.287,P〉0.05）. Conclusion PDCD5 might become a potential molecular marker of early diagnosis of endometrial carcinoma .