摘要
建立乳品中乳铁蛋白高效液相色谱的测定方法.采用pH值为7.0磷酸盐缓冲溶液做为提取剂,C18色谱柱分离,不同浓度的乙腈-0.5 mol/L的氯化钠溶液-三氟乙酸的二元系统进行梯度洗脱.每100 g奶粉（或每100 mL液态奶）中乳铁蛋白添加量为5～10 mg时,回收率大于90％.定量限为0.5 mg/100 g.该方法具有结果准确,操作简便,重复性好等优点,可用于乳品中乳铁蛋白的检测.
A method for the determination of lactoferrin in dairy products by high performance liquid chromatography （HPLC） was developed. Lactoferrin was extracted by the solution of pH 7.0 0.05 mol/L phosphate. The method was performed on C18 column, the analysis was performed by gradient elution using solvent systems acetonitrile-0.5mol/L sodium chloride-TFA. Lactoferrin content was in the range of 5-10 mg/100 g, the recovery was higher than 90%. The limit was 0.5 mg/100 g. The results indicated that this method is accurate, simple and reproducible, and thus suitable for the analysis of lactoferrin in milk products.
出处
《乳业科学与技术》
2014年第4期18-21,共4页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
基金
"十二五"国家科技支撑计划项目(2012BAK17B00)
关键词
高效液相色谱法
乳铁蛋白
肝素免疫亲和柱
high performance liquid chromatography
lactoferrin
HiTrap Heparin HP column
