不同激素配比对卷叶贝母鳞茎诱导效应的影响

Different Ratio of Hormone Effects on Fritillaria cirrhosa Bulb Induction

目的:研究不同激素配比对卷叶贝母组培苗幼叶诱导产生鳞茎的影响,为扩大川贝母药源提供有效途径。方法:以卷叶贝母组培苗幼叶为外植体,通过正交试验法研究了由6-BA、NAA、2,4-D和KT四种植物激素组成的培养基直接诱导鳞茎产生和增殖的影响;实验以鳞茎的诱导率和平均产生鳞茎数为考核指标,并测定了产生鳞茎的生物碱含量。结果:诱导卷叶贝母组培苗幼叶产生鳞茎的最佳培养基配方为MS+6-BA 2.0 mg/L+NAA 1.0mg/L+KT 1.0 mg/L,其中鳞茎诱导率为83.37%,平均产生的鳞茎数为17.27个;并经测定诱导产生的鳞茎中的生物碱含量为0.389%,是野生川贝母的1.64倍。结论:提供了生产组培川贝母药材的简便快捷方法,为合理开发利用卷叶贝母植物资源和提高药材品质奠定基础。

Objective：To study the effects of different hormone induced bulb of Fritillaria cirrhosa seedling leaves,in order to pro- vide an effective way to expand Fritillaria cirrhosa source. Methods：Fritillaria cirrhosa seedling leaves were used as explants, orthogonal test was carried out to study the medium consisting of 6-BA, NAA,2,4-D and KT, and proliferation induced by direct bulb. Induction rate and average number of bulb producing was treated as the assessment indices, and the content of alkaloid of bulb was determined. Results ：The best medium formula for Fritillaria cirrhosa tissue culture seedling was MS ＋ 6-BA 2. 0 mg/L ＋ NAA 1.0 mg/ L ＋ KT 1.0 mg/L,the bulb induction rate was 83.37% and the average number of bulbs was 17. 27. The alkaloid content of induced bulb was 0. 389%, which was 1.64 times that of wild Fritillaria cirrhosa bulb. Conclusion：This study provides a simple and rapid meth- od for the production of tissue culture of Fritillaria cirrhosa bulb. It is useful for reasonable development and utilization of Fritillaria cir- rhosa resources, as well as improvement of the quality of medicinal materials.