藏药康定乌头质量标准研究

Stud on Quality Standard for Aconiti Tatsienensis Radix

目的：建立康定乌头的质量标准。方法：以滇乌头碱、塔拉萨敏为对照品进行康定乌头的薄层鉴别；采用HPLC法测定康定乌头中滇乌头碱的含量。对康定乌头水分、总灰分、酸不溶性灰分、醇溶性浸出物及显微特征进行检测。结果：康定乌头的显微特征性强；薄层色谱分离度好，斑点清晰；水分为10．89％～12．07％（平均值11．48％），总灰分为4．81％～5．00％（平均值4．83％），酸不溶性灰分为1．08％-3．27％（平均值1．81％）；浸出物为18．03％～28．27％（平均值20．46％）；滇乌头碱的含量为0．18％～0．35％（平均值0．23％）。结论：所建方法操作简单，准确可靠，可用于评价康定乌头的质量。

Objective：To establish the quality standard for Aconiti Tatsienensis Radix. Methods：TLC was used to detect yunaconi- tine and talasamine in Aconiti Tatsiensis Radix. HPLC was adopted to determine the content of yunaconitine. Moisture, ash, acid insolu- ble ash and ethanol soluble extractive were determined according the procedures recorded in the Appendix of Chinese Pharmacopoeia （2010 edition）. The microscopic identification was also carried out. Resuhs：Aconiti Tatsienensis Radix had obvious microscopic char- acteristics,and its TLC identification had a good resolution with clear spots. An average content of moisture was 11.48%, ash was 4. 83 % , acid insoluble was 1.81% , ethanol soluble extractive was 20.46% and yunaconitine was 0. 23%. Conclusion：The established standard is acceptable for quality evaluation of Aconiti Tatsienensis Radix.