海藻多糖基嵌段共聚物/纳米金复合物的制备

Preparation of composite of block copolymer/gold nanoparticle based alginate polysaccharide

目的：原位法制备海藻多糖基聚乙烯基吡咯烷酮嵌段共聚物/纳米金复合物（ SA-330-S2-Au）。方法首先合成改性海藻多糖基二硫代酯,并用作RAFT聚合的链转移剂（CTA）,制备海藻多糖基聚乙烯基吡咯烷酮嵌段的共聚物（ SA-330-S2）,然后利用SA-330-S2自组装特性,原位法制备SA-330-S2-Au,并用FT-IR、NMR、DSC、TEM、SEM等方法进行分析表征。结果复合物SA-330-S2-Au中的纳米金粒子大小约10~30 nm,湿态时纳米复合纳米粒子的平均粒径约为54.4 nm,由于复合纳米粒子外壳层的海藻多糖基嵌段共聚物的稳定化作用,SA-330-S2-Au水溶液储存期超过6个月,且有明显荧光。结论 SA-330-S2-Au水溶液稳定且具有荧光特性,有望应用于肿瘤诊疗领域。

Objective The composite was prepared in situ about polyvinyl pyrrolidone block copolymer/nano-gold （ SA-330-S2-Au ） based alginate polysaccharides. Methods The dithioester base alginate polysaccharides was synthesised at first, and then used as a chain transfer agent （ CTA ） for RAFT polymerization, to sythesis polyvinyl pyrrolidone block copolymer （ SA-330-S2 ） based alginate polysaccharides. By the self-assembly properties of SA-330-S2 ,the composite of polyvinyl pyrrolidone block copolymer/nano-gold （ SA-330-S2-Au ） based alginate polysaccharides was prepared in situ, and characterized by using methods such as FT-IR,NMR,DSC,TEM and SEM et al. Results The size of the gold nanoparticles was about 10-30 nm . But the average particle size of composite nanoparticles was about 54.4 nm when it was measured by the method of DLS in wet. Due to the stabilizing effect of block copolymers based seaweed polysaccharide of the shell layer of composite nanoparticles,the storage of SA-330-S2-Au aqueous was more than six months,and there was significant fluorescence. Conclusion The SA-330-S2-Au aqueous solution is very stable with fluorescence properties,and is expected to be used in tumor diagnosis and treatment.