飞龙掌血内生真菌的分离及其抑菌活性研究

Study on isolation of endophytic fungi from Toddalia asiatica and their antimicrobial activity

目的研究药用植物飞龙掌血内生真菌的多样性及其次生代谢产物抑菌活性,旨在寻求新型抑菌活性物质。方法采用内生菌常规分离法对健康飞龙掌血植株的根、茎进行内生真菌的分离鉴定。采用滤纸片法和菌饼法分别对内生真菌及其次生代谢产物进行抑菌试验。结果所得21株飞龙掌血内生真菌,其中有18株对至少1种实验菌有抑菌作用,占总分离菌株的85.7%。有5株内生真菌次生代谢产物对至少1种实验菌有抑菌作用,占总分离菌株的24.0%。结论飞龙掌血内生真菌的分离及其抑菌活性研究,可为寻找新型抑菌物质提供资源。

Objective To investigate endophytic fungi from Toddalia asiatica and antimicrobial effect of their secondary metabolites. Methods Endophytic fungi was isolated from the roots and stems of healthy Toddalia asiatica and identified by conventional method. Antimicrobial tests of the endophytic fungi and their secondary metabolites were carried out by colony disc method and filter paper disc method. Results Among 21 strains of endophytic fungi isolated from Toddalia asiatica, 18 strains （ 85. 7% of the total strains isolated） showed the antimicrobial activity against at least one of 12 experimental bacteria, and the secondary metabolites of 5 strains （ 24. 0% of the total strains isolated ） presented antimicrobial activity against at least one of experimental bacteria. Conclusion The study on endophytic fungi and their secondary metabolites from Toddalia asiatica may provide resources for new antimicrobial products.