江西省2009年-2012年B型流感病毒耐药基因分析

Drug resistance analysis of influenza B virus in Jiangxi from 2009 to 2012

目的分析江西省2009年-2012年B型流感病毒耐药基因位点,掌握其耐药情况,为临床治疗和疾病控制提供参考。方法采集监测医院和疑似流感疫情的流感样病例鼻咽拭子标本进行流感病毒分离,随机选择分离到的B型流感病毒进行核酸提取,采用逆转录-聚合酶链反应(RT-PCR)扩增病毒NA基因后进行核苷酸序列测定,用DNAStar5.0,Mage 3.1生物软件对测序结果进行分析处理,推导出氨基酸序列,进行基因特性分析。结果 24株B型流感病毒NA区域核苷酸序列长度均为1 398 bp,编码466个氨基酸,所有毒株的NA蛋白催化活性位点和辅助位点均未发生氨基酸替换。结论 24株毒株均对流感病毒神经氨酸酶抑制剂药物敏感,但仍应加强对流感病毒的耐药性监测。

Objective To understand the molecular characterization of neuraminidase（NA） genes and NA drug resistance of influenza B virus in Jiangxi from 2009 to 2012,so as to provide reference for clinical treatment and diseases control.Methods The nasopharyngeal swabs specimens were collected from influenza-like cases of influenza monitoring hospitals for influenza B virus isolation. Twenty-four strains of influenza B virus were randomly selected for virus RNA extraction. Then fragments of NA genes were amplified by one-step RT-PCR and sequenced. The sequencing results were analyzed with the software DNAStar 5. 0 and Mage 3. 1. Results The NA gene of the 24 influenza B strains was consisted of 1 398 nucleotides,encoding 466 amino acids. All strains were not found mutations in catalytic active sites and associated sites of NA gene. Conclusion The 24 influenza B virus strains were all sensitive to neuraminidase-inhibitor drugs,but continuous drug resistance surveillance is still necessary for control and prevention of influenza.