基于响应面法分析温度和盐度及其交互作用对大菱鲆幼鱼抗氧化酶活性的影响

Effects of temperature,salinity and their interaction on the activities of antioxidant enzymes of juvenile turbot Scophthalmus maximus based on response surface methodology

为了分析温度和盐度及其交互作用对大菱鲆（Scophthalmus maximus）抗氧化酶活性的影响,作者采用中心组合设计和响应曲面法研究温度（17~23℃）和盐度（20~40）对大菱鲆幼鱼血清超氧化物歧化酶（SOD）活力的效应。结果表明：温度对大菱鲆幼鱼血清SOD活力的线性效应极显著（P〈0.01）,累积效应不显著（P〉0.05）；盐度对大菱鲆幼鱼肠道 SOD 活力线性效应显著（P〈0.05）,累积效应极显著（P〈0.01）；温度和盐度对SOD 活力的互作效应达到显著水平（P〈0.05）。响应曲面法分析表明,随着温度和盐度的增大, SOD的活力基本上均呈先降后升的趋势。建立SOD活力与温度、盐度及其温度-盐度互作效应的模型方程Y=74.76-9.53 X1＋5.31 X2＋1.8 X1 X2＋5.31 X12＋10.53 X22,并据此预测大菱鲆幼鱼血清SOD的活力。对所建模型进行优化,得到SOD活性最低的理论最佳条件为：温度21.84℃,盐度27.97,在此条件下SOD活性值为70.0463 U/mg。

The central composite design and response surface methodology were used in the experiment to examine the combined effect of temperature （17-23 ℃） and salinity （20-40） on serum Superoxide Dismutase （SOD） activities of turbot juveniles. The results showed that the linear of temperature on the serum SOD activity was highly significant （P 〈 0.01）, and the quadratic was not significant （P 〉 0.05）. The linear of salinity on the serum SOD activity was significant （P 〈 0.05）, and the quadratic was highly significant （P 〈 0.01） in turbot juveniles. The interactive effect between temperature and salinity on SOD activity was significant （P〈0.05）. By using the response surface method, the SOD activity varied mainly with increasing temperature and salinity in a curvilinear way. The model equation of SOD activity towards temperature, salinity and the interactive effect between temperature and salinity was established （Y= 74.76-9.53 ）（1 ＋5.31 ）（2 ＋ 1.8 ）（1 ）（2 ＋ 5.31 ）（12＋10.53 ）（22）. The model equation could be practically used for forecasting the serum SOD activity in turbot juveniles. Via applying statistical optimization of the response of interest, the result showed that the minimum SOD activity was 70.0463 U/mg under the optimal conditions with a temperature of 21.84℃ and a salinity of 27.97.