CAV-1在结直肠癌患者肠黏膜中的表达及其意义探讨

CAV-1 expression in the mucosal cells of colorectal cancer and its role in the clinical practice

[目的]研究质膜微囊蛋白1(caveolin-1,CAV-1)在结直肠癌(colorectal cancer,CRC)患者大肠黏膜中的表达,并初步探讨其临床意义。[方法]在肠镜室募集无器质性肠道疾病患者12例、结直肠癌患者16例,通过结肠镜分别在无器质性肠道疾病患者的乙状结肠处和结直肠癌患者的病变明显处取材,每例取3块。获取的标本经免疫组织化学染色,检测CAV-1在结肠黏膜中的表达。[结果]免疫组织化学法检测CAV-1在无器质性肠道疾病患者和结直肠癌结肠黏膜中的表达发现,CAV-1蛋白阳性染色主要位于胞浆,胞核少见或未见染色;在无器质性肠道疾病患者的结肠黏膜组织胞浆中仅有少量阳性染色点,而CAV-1在结直肠癌组织中的表达明显,染色呈现颗粒状。无器质性肠道疾病患者组CAV-1表达的阳性指数为(12.87±2.76),结直肠癌组的表达明显增强,其阳性指数为(34.42±5.44),较无器质性肠道疾病患者表达明显增强(P<0.01)。[结论]CAV-1在直结肠癌黏膜中高表达,CAV-1是结直肠癌发病的促使因子。

[Objective]To investigate the expression of caveolin I（CAV-1） in the mucosal cells of color ectal carcinoma and its clinical significance. [Methods]Patients were collected in the colonoscopy chamber, including 12 cases without organic bowel diseases and 16 with colorectal cancers. The mucosal biopsy was taken respectively in the sigmoid colon of patients without organic bowel diseases and in lesions of serious tissue of patients with colorectal cancer by eolonoscopy. Through the immunohistochemical staining, the expression of CAV-1 was detected in the sigmoid colon and the lesions of serious tissue. [Results]CAV 1 positive staining was mainly located in cytoplasm and nucleus had rare or no staining. Only a few positive points were observed in the cytoplasm of colonic mucosa tissue from patients without organic bowel disea ses, while CAV 1 expression in the mueosa of colorectal cancer was significantly stained as granules. Compared with the group of no organic bowel disease patients which the positive index of CAV-1 expression was 12.87 ±2.76 ,the positive index was significantly enhanced in colorectal cancer group （34.42±5.44, P〈0.01）. [Conclusion]The expression of CAV-1 in the mucosa of colorectal carcinoma was elevated. CAV-1 may play a promoting role in the occurrence of colorectal carcinoma.