ARTPQ对雌性大鼠生殖内分泌影响

Effects of ARTPQ on reproductive and endocrine system of female rats

目的研究新一代青蒿素抗疟药物ARTPQ重复给药对雌性大鼠生殖内分泌的影响。方法 Wistar大鼠随机分4组,对照组和ARTPQ低、中、高剂量组(35、70、120 mg/kg),每组15只,ig给药14 d。给药后每天测量大鼠动情周期,给药结束后采集大鼠血液,Elisa方法检测大鼠血液雌激素、肾上腺皮质激素水平,肉眼观察各脏器变化及称量大鼠子宫、卵巢、肾脏及肾上腺的质量。结果连续给药3 d后大鼠动情周期紊乱(维持于动情后期或间期),大鼠子宫未见有扩张,平均质量低于对照组。ARTPQ低、中、高剂量组大鼠肾上腺系数高于对照组(P<0.05、0.01)。ARTPQ高剂量组大鼠雌激素水平低于对照组(P<0.05),而ARTPQ低、中、高剂量组大鼠肾上腺素水平高于对照组(P<0.05、0.01)。结论 ARTPQ作用于肾上腺,肾上腺增生,肾上腺皮质激素的水平升高,雌激素水平降低,表现为大鼠发情周期的紊乱。

To study the ARTPQ toxicity in reproduction and endocrinology by repeated administration toxicity. Methods Wistar rats were divideded into four groups randomly, they were control and ARTPQ low-, med- and high-dose （35, 70, and 120 mg/kg） groups, which were ig administered for 14 d. Tbe estrous cycles of rats were measured daily after administration. At the end of the administration, blood was collected and measured by Elisa for the content of estrogen and cot-tin in rats： The changes of visceral organ were observed by eye; And uterus, ovaries, kidneys and adrenals in rats were weighed. Results The estrous cycles of rats were disordered （maintained in postestrus and anestrus） after 3 d of continuous administration, there was no expansion of the rat uterus, the average weight was lower than that in the control group. The factors of adrenal in ARTPQ low-, med-, and high-dose groups were higher than those in the control group （P 〈0.05, P 〈 0.01）.The contents of estrogen in ARTPQ high-dose group were higher than those in the control group （P 〈 0.05）, and the contents of cortin in ARTPQ low-, med-, and high-dose groups were higher than those in the control group （P 〈 0.05, P 〈 0.01）. Conclusion ARTPQ acts on the adrenal glands, adrenal hyperplasia, and adrenal hormone levels rise, while estrogen levels lower, so that the rat estrous cycle disorders.