摘要
目的建立以HPLC法测定六味木香胶囊中木香烃内酯和去氢木香内酯的方法。方法色谱柱为VP-ODS柱(250mm×4.6 mm,5μm),流动相为甲醇-水(72∶28),检测波长为225 nm,柱温为25℃,体积流量为1.0 mL/min。结果木香烃内酯、去氢木香内酯进样量分别在203.12~2 031.20、207.84~2 078.40 ng与峰面积积分值呈良好的线性关系(r=0.999 9);平均回收率分别为101.1%、101.2%,RSD分别为0.48%、0.73%(n=6)。结论建立的方法简便、准确、重复性好、专属性强,可用于该制剂的质量控制和评价。
Objective To establish an HPLC method for the determination of costunolide and dehydrocostuslactone in Liuwei Muxiang Capsules. Methods The determination was performed on VP-ODS (250 mm ×4.6 mm, 5 μm) with column temperature set at 25℃. The mobile phase consisted of methanol-0.4% phosphoric acid water (72 : 28) at a flow rate of 1.0 mL/min. The detection wavelength was set at 225 nm. Results The linear range of costunolide and dehydrocostuslactone was 203.12--2 031.20 ng and 207.84--2 078.40 ng (r = 0.999 9), with average recovery at 101.1% and 101.2%, RSD = 0.48% and 0.73% (n = 6). Conclusion The method proved is simple, accurate, reproducible, and specific, and it can be used for the quality control and evaluation of Liuwei Muxiang Capsules.
出处
《中草药》
CAS
CSCD
北大核心
2014年第16期2349-2351,共3页
Chinese Traditional and Herbal Drugs