气相色谱内标法测定油脂中脂肪酸绝对含量

Determination of Absolute Triglyceride Content in Oil and Fat by Gas Chromatography Internal Standard Method

以十七酸甲酯为内标物,求取了十七酸甲酯相对于棕榈酸甲酯、硬脂酸甲酯、油酸甲酯、亚油酸甲酯和亚麻酸甲酯的校正因子。通过气相色谱面积归一化法对油脂中脂肪酸绝对含量进行了测定,所得结果准确,具有较强的实用价值。

Using methyl heptadecanoate as internal standard, relative con＇eetion factors were acquired between methyl heptadeeanoate and methyl palmitatek, methyl stearate, methyl oleate, methyl linoleate and Methyl linolenate. Absolute triglyceride content of soybean oil was delermined by gas chromatography area normalization method. Result was accurate, determination method was practical.