高产β-胡萝卜素的红酵母培养条件的优化

The Fermentation Technology Optimization with High β-carotene by Red Yeast

本实验以红酵母菌株PTH-1、SG-2、TR-3、SG-4、TR-5、TR-6为实验材料,探讨了不同碳源、氮源、pH值、培养温度对红酵母生物量及产β-胡萝卜素的影响;在单因素优化实验基础上进行正交试验,实验结果表明产β-胡萝卜素最优条件为:葡萄糖8%,蛋白胨1.5%,酵母浸粉0.5%,磷酸二氢钾0.1%,硫酸镁0.1%,pH 5.5,30℃;菌株经过84h摇瓶培养后各菌株的生物量、β-胡萝卜素产量都有了不同程度的提高。其中,菌株TR-6生物量和β-胡萝卜素产量分别达到了12.31g/L和567.42μg/g,比优化前提高了89.3%和16.51%,达到了较好的效果,其他菌株生物量和β-胡萝卜素也有了较大程度的提高;本实验结果为后续应用研究提供了良好的数据平台。

Red yeast strains PTH-1, SG-2, TR-3, SG-4, TR-5, TR-6 were investigated, Effect of different car- bon source, nitrogen source, culture temperature and pH value etc. on biomass and β-earotene production of strain Rhodotorula sp. were investigated, and the optimal combination was received through the single variable test and orthogonal test. The result showed that the optimal fermentation condition were as follows： glucose 8%, peptonel.5%, yeast powder 0.5%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, pH 5.5, 30℃, fermentation time 84h. After optimization of TR-6 strain of biomass and beta carotene production were 12.31g/L and 567.42μg/g which had increased by 89.3% and 16.51% compared with its original output 6.98% and 487.25μg/g, and the biomass and β-carotene production of other strains had improved also. So these results had provided a good platform of the data for the subsequent application research.