中华鳖养殖水体光合细菌的分离、鉴定与固化

Isolation,Identification and Immobilization of Photosynthetic Bacteria from Chinese Soft Shelled Turtle Trionyx sinensis Culture Waters

对自中华鳖浅红色养殖水体中分离出的1株光合细菌进行菌体和菌落的形态观察、生理生化特征分析及16SrDNA序列相似性分析,将鉴定出的菌株用海藻酸钠法固化,在实验室规模开展对中华鳖养殖水体的处理试验。试验结果表明,菌落为深红色圆形菌落,菌体短杆状,革兰氏阴性,能运动,为兼性厌氧菌。鉴定该菌株为类球红细菌。对固化细菌的包埋材料—琼脂、海藻酸钠、聚乙烯醇+海藻酸钠进行比较,其中3%海藻酸钠溶液与4%CaCl2溶液交联,成球质量好,操作简便,被选作包埋材料。水处理结果显示,6d时游离菌液组和固化细菌组的化学需氧量去除率分别为42.2%、65.1%,氨氮去除率分别为43.1%、62.1%,固化类球红细菌明显提高了水质处理效果。

A strain of photosynthetic bacterium was isolated from the “red water” in Chinese soft shelled turtle Trionyx sinensis culture ponds ,and were identified and immobilized by morphological ,physiological and biochemical characteristics and 16S rDNA sequencing .It was found that the bacterium with dull red round and regular colonies was short rod-shaped ,gram-negative ,motile ,and cultative anaerobic ,and i-dentified as Rhodobacter sphaeroides .The immobilized material screening showed that good quality cross-linked microsphere was observed in 3% sodium alginate solution with 4% CaCl2 solution .The Chinese soft shelled turtle culture water treated by immobilized R .sphaeroides in a laboratory modulated experiment showed that 42 .2% of COD and 43 .1% of ammonia nitrogen were eliminated from the waters in free cells group and 65 .1% of COD ,and 62 .1% of ammonia nitrogen in the immobilized cells ,indicating that immo-bilization of the bacteria improves the treatment effect of aquaculture waste waters .The findings will pro-vide reference to the treatment of wastewater produced by Chinese soft shelled turtle or other cultured ani-mals .