赤红球菌CGMCC3090生物转化肉桂腈合成肉桂酰胺的工艺

Biotransformation of Cinnamonitrile into Cinnamamide with Rhodococcus ruber CGMCC3090

针对目前肉桂酰胺工业生产采用的肉桂酸和二氯亚砜、浓氨水两步化学反应工艺存在安全性较低、产物分离纯化困难等问题,建立并优化了赤红球菌CGMCC3090催化肉桂腈生成肉桂酰胺的转化工艺.研究表明:底物肉桂腈浓度为1.5,mol/L,赤红球菌菌体质量浓度为2.6,g/L,在30,℃、pH 7.5条件下,转化5,h后转化率可达100%,具有产物纯度高、无副产物肉桂酸生成的优势,为赤红球菌CGMCC3090生物转化合成肉桂酰胺的工艺放大和生产性应用奠定了良好基础.

At present,the method of cinnamamide production consists of two steps by using cinnamic acid,thionyl chloride and strong ammonia water. This chemical process has less security,product separation and purification are difficult and there are some other problems. To solve these problems,the process of biotransformation of cinnamonitrile into cinnamamide us-ing Rhodococcus ruber CGMCC3090,was established. It was found that the conversion after 5,h can reach 100%when the cinnamonitrile was 1.5,mol/L,the cell concentration was 2.6,g/L,the temperature 30,℃ and pH 7.5. This process has the advantage of high product purity without formation of cinnamic acid. It has laid a good foundation for the scale-up process and practical production of cinnamamide biosynthesis with R. ruber CGMCC3090.