丹参酮ⅡA下调凋亡抑制蛋白XIAP诱导人肝癌细胞凋亡的研究

The Research of Down Regulation of Inhibitor of Apoptosis Protein XIAP and Inducing Human Hepatocellular Carcinoma Apoptosis by Tanshinone ⅡA

目的:探讨丹参酮ⅡA对肝癌细胞中凋亡抑制蛋白XIAP的影响。方法:通过MTT实验、流式细胞术、细胞形态观察检测丹参酮ⅡA对细胞增殖和凋亡的影响,通过蛋白免疫印迹的方法检测丹参酮ⅡA对细胞中凋亡抑制蛋白XIAP、凋亡蛋白caspase-3以及PARP蛋白的影响。结果:MTT、细胞形态结果显示丹参酮ⅡA对SMMC-7721细胞的生长抑制作用成时间和剂量依赖性,免疫印迹结果显示,0.5μg/mL丹参酮ⅡA作用肝癌细胞24 h后,caspase-3前体、凋亡抑制蛋白XIAP表达明显下调,活化的caspase-3、剪切形式的PARP含量显著增加。结论:丹参酮ⅡA下调凋亡抑制蛋白XIAP,活化caspase-3,促进PARP剪切诱导肝癌细胞凋亡。

To investigate the effect of inhibitor of apoptosis protein XIAP in hepatocellular carcinoma cells treated with TanshinoneⅡA.Method：Effect of TanshinoneⅡA on cell proliferation and apoptosis were determined by MTT test,flow cytometry and cell morphology observation.The effects of TanshinoneⅡA on inhibitor of apoptosis protein XIAP,apoptosis related proteins procaspase-3 and PARP protein in cells were measured by Western blot.Result：MTT,cell morphology results showed that TanshinoneⅡA inhibited the growth of SMMC-7721 cells in a dose and time dependent manner,Western blotting results showed that 0.5μg/mL tanshinoneⅡA in hepatocellular carcinoma cells after 24 h,Caspase-3 precursor,inhibitor of apoptosis protein XIAP expression were down regulated,the content of PARP,caspase-3 splicing activation increased significantly.Conclusion：Tanshinone ⅡA can down regulate XIAP protein,activate caspase-3 and promote PARP shear,and induce apoptosis of hepatocellular carcinoma cells.