不同PCR扩增试剂盒检验血样DNA的检验结果对比研究

Comparative Study between Test Results of Different PCR Amplification Kits in Testing Blood Sample DNA

目的:讨论研究Profiler PlusTM、IdentifilerTM以及Powerplex16扩增试剂盒用于检验血样DNA检验结果的差异,并研究其扩张不平衡和基因丢失现象的发生几率。方法:选取150例完全无血缘关系的个体作为研究对象并采集其血样,分别使用两种扩增试剂盒进行检验。对所得不同结果的同一对象再使用3种扩增试剂盒进行检验。将检验所得结果进行比较。结果:3种试剂盒检测的等位基因缺失率比较差异无统计学意义(P>0.05)。Profiler PlusTM检测扩张不平衡率显著高于其余两种试剂盒(P<0.05)。结论:使用PCR扩增试剂盒对血样DNA进行检测时,会出现不同位置的异常基因,可表现为基因缺失及扩增不平衡。但Profiler PlusTM检验扩增不平衡发生率显著高于其余两种试剂盒。在实际生活对血样DNA进行检测时,除需准备主要检测扩增试剂盒外,还需准备其他不同类备用试剂盒用于互相验证及对比,尽量降低基因等位缺失及扩张不平衡发生率。

To discuss and study the difference between testing results of Profiler PlusTM,IdentifilerTM and Powerplex16 amplification kits in testing blood sample DNA,and study the occurrence rate of amplification imbalance and gene deletion phenomenon.Method：150 individuals who had no genetic connection with each other were chosen as research objects,and their blood samples were collected and tested by 2 different amplification kits. Objects with 2 different results were tested again by the third kit. All testing results were compared.Result：There were no significant differences between the 3 kits on allelic gene deletion（P〈0.05）. Amplification imbalance rate of Profiler PlusTM was obviously higher than the other 2 kits（P〈0.05）. Conclusion：When testing blood sample DNA with different PCR amplification kits, there might be abnormal genes of different positions which might manifested as gene deletion and amplification imbalance. But occurrence rate of amplification imbalance of testing result of Profiler PlusTM was obviously higher than the other 2 kits. In practical life,when testing blood sample DNA,besides major amplification kits,other different sorts of spare kits should be prepared for verification and comparison to reduce occurrence rates of allelic gene deletion and amplification imbalance.