高原习服过程淋巴细胞线粒体DNA含量及氧化损伤变化规律

Changes in contents of leukocyte mitochondrial DNA and oxidative damage during acclimatization to highland

目的观察世居平原男性青年高原习服各时程淋巴细胞线粒体能量代谢、线粒体DNA(mtDNA)拷贝数及其氧化损伤的动态变化规律。方法 27名世居平原武警新兵急进高原,分别在移居高原3、7、90 d检测淋巴细胞线粒体膜电位、mtDNA中8-oxodG含量、mtDNA拷贝数和PGC-1α蛋白表达。结果与平原阶段比较,移居高原3 d和7 d,膜电位显著降低(P<0.05),8-oxodG含量,mtDNA拷贝数和PGC-1α表达显著升高(P<0.05);移居高原90 d,mtDNA拷贝数和PGC-1α表达显著降低(P<0.05)。移居高原90 d与移居高原7 d比较,膜电位显著升高(P<0.05),8-oxodG含量,mtDNA拷贝数和PGC-1α表达显著降低(P<0.05)。结论高原习服初期淋巴细胞能量重构主要依赖于线粒体数量增加,而在高原习服后期主要依赖于单个线粒体健康程度及能量代谢水平提高。

Objective To study the changes in mitochondrial bioenergetics, mitochondrial DNA （mtDNA） and oxidative dam- age in leukocytes during acclimatization to highland hypoxia. Methods Twenty-seven lowlanders quickly entered plateau. Leukocyte mitochondrial membrane potential, 8-oxodG in mtDNA, mtDNA copies and PGC-lct protein expression were measured in plain and plateau at 3 d,7 d,90 d after entry. Results As compared with plain group, membrane potential was significantly decreased （ P 〈 0.05 ） ; 8-oxodG amounts, mtDNA copies and PGC-1 c~ expression were markedly increased at 3 d and 7 d （ P 〈 0.05 ）. And at 90 d, mtDNA copies and PGC-1 o~ expression were significantly decreased （ P 〈 0.05 ）. As compared with 7 d group, membrane potential was significantly increased （P 〈 0.05 ） ; 8-oxodG amounts, mtDNA copies and PGC-lc~ expression were markedly decreased at 90 d （P 〈 0.05 ）. Conclusions In the early phase of acclimatization to highland, leukocyte bioenergetics remodeling mainly depends on in- creased mitochondrial content. In the later phase of acclimatization to highland, leukocyte bioenergetics remodeling mainly depends on elewited level of single mitochondrial health and energy metabolism.